TY - JOUR
T1 - Protective Effects of Lycopene Against Ultraviolet B-Induced Photodamage
AU - Fazekas, Zsuzsanna
AU - Gao, Dayuan
AU - Saladi, Rao N.
AU - Lu, Yuhun
AU - Lebwohl, Mark
AU - Wei, Huachen
N1 - Funding Information:
This work was supported by a fellowship grant awarded to Dr. Zsuzsanna Fazekas (fellow) and Dr. Huachen Wei (mentor) from the Cancer Research Foundation of America. Address correspondence to H. Wei, Department of Dermatology, Mount Sinai School of Medicine, 1425 Madison Avenue, New York, NY 10029. Phone: 212–659–9533. FAX: 212–348–7434. E-mail: [email protected].
PY - 2003
Y1 - 2003
N2 - Lycopene, an acyclic hydrocarbon carotenoid found in tomatoes and their products, is a well-established potent antioxidant, and its anticancer properties have been shown in cultured cells and animal models. We investigated the protective effects of two concentrations of topical lycopene against acute ultraviolet B (UVB)-induced photodamage. Application of lycopene dose dependently inhibited UVB-induced ornithine decarboxylase (P < 0.05) and myeloperoxidase (P < 0.05) and significantly reduced bifold skin thickness (P < 0.05). Immunohistochemical staining revealed increased active caspase-3 of apoptotic pathway in the UVB-exposed group compared with the unexposed control Application of topical lycopene prevented the cleavage of caspase-3. UVB irradiation completely diminished proliferating cell nuclear antigen (PCNA), and the untreated skin maintained positively stained cells throughout the basal epidermis. Topical application of lycopene significantly reversed UVB-induced PCNA inhibition, and normal PCNA staining was restored in the lycopene-treated skin. Our results suggest that topical lycopene is able to exert its protective effects against acute UVB-induced photodamage. Furthermore, it may act as a preventative agent via inhibition of epidermial ornithine decarboxylase activity, reducing inflammatory responses, maintaining normal cell proliferation, and possibly preventing DNA damage as indicated by blocking the necessitating step of apoptosis following UVB injury.
AB - Lycopene, an acyclic hydrocarbon carotenoid found in tomatoes and their products, is a well-established potent antioxidant, and its anticancer properties have been shown in cultured cells and animal models. We investigated the protective effects of two concentrations of topical lycopene against acute ultraviolet B (UVB)-induced photodamage. Application of lycopene dose dependently inhibited UVB-induced ornithine decarboxylase (P < 0.05) and myeloperoxidase (P < 0.05) and significantly reduced bifold skin thickness (P < 0.05). Immunohistochemical staining revealed increased active caspase-3 of apoptotic pathway in the UVB-exposed group compared with the unexposed control Application of topical lycopene prevented the cleavage of caspase-3. UVB irradiation completely diminished proliferating cell nuclear antigen (PCNA), and the untreated skin maintained positively stained cells throughout the basal epidermis. Topical application of lycopene significantly reversed UVB-induced PCNA inhibition, and normal PCNA staining was restored in the lycopene-treated skin. Our results suggest that topical lycopene is able to exert its protective effects against acute UVB-induced photodamage. Furthermore, it may act as a preventative agent via inhibition of epidermial ornithine decarboxylase activity, reducing inflammatory responses, maintaining normal cell proliferation, and possibly preventing DNA damage as indicated by blocking the necessitating step of apoptosis following UVB injury.
UR - http://www.scopus.com/inward/record.url?scp=2342507131&partnerID=8YFLogxK
U2 - 10.1207/s15327914nc4702_11
DO - 10.1207/s15327914nc4702_11
M3 - Article
C2 - 15087271
AN - SCOPUS:2342507131
SN - 0163-5581
VL - 47
SP - 181
EP - 187
JO - Nutrition and Cancer
JF - Nutrition and Cancer
IS - 2
ER -