@article{085b0e02ce5b4559af2f0f008f80b94a,
title = "Prolonged evolution of the memory B cell response induced by a replicating adenovirus-influenza H5 vaccine",
abstract = "Induction of an antibody response capable of recognizing highly diverse strains is a major obstacle to the development of vaccines for viruses such as HIV and influenza. Here, we report the dynamics of B cell expansion and evolution at the single-cell level after vaccination with a replication-competent adenovirus type 4 recombinant virus expressing influenza H5 hemagglutinin. Fluorescent H1 or H5 probes were used to quantitate and isolate peripheral blood B cells and their antigen receptors. We observed increases in H5-specific antibody somatic hypermutation and potency for several months beyond the period of active viral replication that was not detectable at the serum level. Individual broad and potent antibodies could be isolated, including one stem-specific antibody that is part of a new multidonor class. These results demonstrate prolonged evolution of the B cell response for months after vaccination and should be considered in efforts to evaluate or boost vaccine-induced immunity.",
author = "Kenta Matsuda and Jinghe Huang and Tongqing Zhou and Zizhang Sheng and Kang, {Byong H.} and Elise Ishida and Trevor Griesman and Sarah Stuccio and Lyuba Bolkhovitinov and Wohlbold, {Teddy J.} and Veronika Chromikova and Alberto Cagigi and Kwanyee Leung and Sarah Andrews and Cheung, {Crystal S.F.} and Pullano, {Alyssa A.} and Jason Plyler and Cinque Soto and Baoshan Zhang and Yongping Yang and {Gordon Joyce}, M. and Yaroslav Tsybovsky and Adam Wheatley and Narpala, {Sandeep R.} and Yicheng Guo and Sam Darko and Bailer, {Robert T.} and April Poole and {Jason Liang}, C. and Jon Smith and Jeff Alexander and Marc Gurwith and Migueles, {Stephen A.} and Koup, {Richard A.} and Hana Golding and Surender Khurana and McDermott, {Adrian B.} and Lawrence Shapiro and Florian Krammer and Kwong, {Peter D.} and Mark Connors",
note = "Funding Information: We thank members of the Vaccine Research Center for discussions of the manuscript. We thank S. Strohmeier and F. Amanat for technical assistance with protein expression in the Krammer laboratory. J.A., J.S., and M.G. were employed by PaxVax Inc. (Redwood City, CA 94065, USA), which was acquired by Emergent BioSolutions. J.S. is currently employed by ClearPath Vaccines (Rockville, MD 20855, USA). M.G. is currently employed by Verndari Inc. (Sacramento, CA 95817, USA) and Jiangsu Atom Bioscience and Pharmaceutical Co., Ltd. (Zhenjiang, China). J.H. is currently employed at Fudan University, Shanghai City, China This work was supported by the Intramural Research Programs of the Vaccine Research Center and of the National Institute of Allergy and Infectious Diseases (NIAID), NIH. Use of sector 22 (Southeast Region Collaborative Access team) at the Advanced Photon Source was supported by the U.S. Department of Energy, Basic Energy Sciences, Office of Science, under contract number W-31-109-Eng-38. Work by Leidos was supported, in part, with federal funds from the Frederick National Laboratory for Cancer Research, NIH, under contract HHSN261200800001E. Funding to the Krammer laboratory was provided by NIAID grant U19 AI109946 and the Centers of Influenza Virus Research and Surveillance (CEIRS) contract HHSN272201400008C. Publisher Copyright: Copyright {\textcopyright} 2019 The Authors.",
year = "2019",
month = apr,
day = "19",
doi = "10.1126/sciimmunol.aau2710",
language = "English",
volume = "4",
journal = "Science immunology",
issn = "2470-9468",
publisher = "American Association for the Advancement of Science",
number = "34",
}