@article{7c2c5a89ce31438ab7ee75b7a95d7d19,
title = "Programming human pluripotent stem cells into white and brown adipocytes",
abstract = "The utility of human pluripotent stem cells is dependent on efficient differentiation protocols that convert these cells into relevant adult cell types. Here we report the robust and efficient differentiation of human pluripotent stem cells into white or brown adipocytes. We found that inducible expression of PPARG2 alone or combined with CEBPB and/or PRDM16 in mesenchymal progenitor cells derived from pluripotent stem cells programmed their development towards a white or brown adipocyte cell fate with efficiencies of 85%-90%. These adipocytes retained their identity independent of transgene expression, could be maintained in culture for several weeks, expressed mature markers and had mature functional properties such as lipid catabolism and insulin-responsiveness. When transplanted into mice, the programmed cells gave rise to ectopic fat pads with the morphological and functional characteristics of white or brown adipose tissue. These results indicate that the cells could be used to faithfully model human disease.",
author = "Tim Ahfeldt and Schinzel, {Robert T.} and Lee, {Youn Kyoung} and David Hendrickson and Adam Kaplan and Lum, {David H.} and Raymond Camahort and Fang Xia and Jennifer Shay and Rhee, {Eugene P.} and Clish, {Clary B.} and Deo, {Rahul C.} and Tony Shen and Lau, {Frank H.} and Alicia Cowley and Greg Mowrer and Heba Al-Siddiqi and Matthias Nahrendorf and Kiran Musunuru and Gerszten, {Robert E.} and Rinn, {John L.} and Cowan, {Chad A.}",
note = "Funding Information: The authors would like to thank T. Holm, A. Foudi and E. Hanson for critical reading, insight and suggestions; M. Henderson, J. Hom, K. Hom, I. Pomerantseva, A. Tseng and K. Kulig for their technical help; L. Prickett-Rice and K. Folz-Donahue of the HSCI-CRM Flow Cytometry Core Facility; D. Lieber and V. Mootha for assistance with cellular bioenergetic measurements; and J. Truelove and R. Weissleder for assistance with PET–CT imaging. T. Ahfeldt was supported by the Roberto and Allison Mignone Fund for Stem Cell Research. This work was financially sponsored in part by the Harvard Stem Cell Institute, Hoffman-La Roche and the Stowers Medical Institute.",
year = "2012",
month = feb,
doi = "10.1038/ncb2411",
language = "English",
volume = "14",
pages = "209--219",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "2",
}