TY - JOUR
T1 - Profiling TRA-1-81 antigen distribution on a human embryonic stem cell
AU - Qiu, Dengli
AU - Xiang, Jialing
AU - Li, Zhaoxia
AU - Krishnamoorthy, Aparna
AU - Chen, Liaohai
AU - Wang, Rong
N1 - Funding Information:
The authors thank Dr. Bin Shi at ANL for her acquisition of SEM images of AFM probes and Mr. Yang Ha’s help in data processing. This research was supported by NIH (R01 NS047719) and a seed grant from the Pritzker Institute of Biomedical Science and Engineering at IIT.
PY - 2008/5/2
Y1 - 2008/5/2
N2 - Human embryonic stem (hES) cells hold great promise in regenerative medicine. Although hES cells have unlimited self-renewal potential, they tend to differentiate spontaneously in culture. TRA-1-81 is a biomarker of undifferentiated hES cells. Quantitative characterization of TRA-1-81 expression level in a single cell helps capture the "turn-on" signal and understand the mechanism of early differentiation. Here, we report on our examination of TRA-1-81 distribution and association on a hES cell membrane using an atomic force microscope (AFM). Our results suggest that aggregated distribution of TRA-1-81 antigen is characteristic for undifferentiated hES cells. We also evaluated the TRA-1-81 expression level at ∼17,800 epitopes and ∼700 epitopes per cell on an undifferentiated cell and a spontaneously differentiated cell, respectively. The method in this study can be adapted in examining other surface proteins on various cell types, thus providing a general tool for investigating protein distribution and association at the single cell level.
AB - Human embryonic stem (hES) cells hold great promise in regenerative medicine. Although hES cells have unlimited self-renewal potential, they tend to differentiate spontaneously in culture. TRA-1-81 is a biomarker of undifferentiated hES cells. Quantitative characterization of TRA-1-81 expression level in a single cell helps capture the "turn-on" signal and understand the mechanism of early differentiation. Here, we report on our examination of TRA-1-81 distribution and association on a hES cell membrane using an atomic force microscope (AFM). Our results suggest that aggregated distribution of TRA-1-81 antigen is characteristic for undifferentiated hES cells. We also evaluated the TRA-1-81 expression level at ∼17,800 epitopes and ∼700 epitopes per cell on an undifferentiated cell and a spontaneously differentiated cell, respectively. The method in this study can be adapted in examining other surface proteins on various cell types, thus providing a general tool for investigating protein distribution and association at the single cell level.
KW - Affinity mapping
KW - Human embryonic stem cell
KW - Protein distribution
KW - TRA-1-81
UR - http://www.scopus.com/inward/record.url?scp=40849119667&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.02.102
DO - 10.1016/j.bbrc.2008.02.102
M3 - Article
C2 - 18313397
AN - SCOPUS:40849119667
SN - 0006-291X
VL - 369
SP - 735
EP - 740
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -