Profiling Microbiota from Multiple Sites in the Respiratory Tract to Identify a Biomarker for PM_2.5 Nitrate Exposure-Induced Pulmonary Damages

The microbiota present in the respiratory tract (RT) responds to environmental stimuli and engages in a continuous interaction with the host immune system to maintain homeostasis. A total of 40 C57BL/6 mice were divided into four groups and exposed to varying concentrations of PM_2.5 nitrate aerosol and clean air. After 10 weeks of exposure, assessments were conducted on the lung and airway microbiome, lung functions, and pulmonary inflammation. Additionally, we analyzed data from both mouse and human respiratory tract (RT) microbiomes to identify possible biomarkers for PM_2.5 exposure-induced pulmonary damages. On average, 1.5 and 13.5% inter-individual microbiome variations in the lung and airway were explained by exposure, respectively. In the airway, among the 60 bacterial OTUs (operational taxonomic units) > 0.05% proportion, 40 OTUs were significantly affected by PM_2.5 exposure (FDR ≤ 10%). Further, the airway microbiome was associated with peak expiratory flow (PEF) (p = 0.003), pulmonary neutrophil counts (p = 0.01), and alveolar 8-OHdG oxidative lesions (p = 0.0078). The Clostridiales order bacteria showed the strongest signals. For example, the o_Clostridiales;f_;g_ OTU was elevated by PM_2.5 nitrate exposure (p = 4.98 × 10^-5) and negatively correlated with PEF (r = −0.585 and p = 2.4 × 10^-4). It was also associated with the higher pulmonary neutrophil count (p = 8.47 × 10^-5) and oxidative lesion (p = 7.17 × 10^-3). In human data, we confirmed the association of airway Clostridiales order bacteria with PM_2.5 exposure and lung function. For the first time, this study characterizes the impact of PM_2.5 exposure on the microbiome of multiple sites in the respiratory tract (RT) and its relevance to airflow obstructive diseases. By analyzing data from both humans and mice, we have identified bacteria belonging to the Clostridiales order as a promising biomarker for PM_2.5 exposure-induced decline in pulmonary function and inflammation.