TY - JOUR
T1 - Profiling antibodies to class II HLA in transplant patient sera
AU - McMurtrey, Curtis
AU - Lowe, Dave
AU - Buchli, Rico
AU - Daga, Sunil
AU - Royer, Derek
AU - Humphrey, Alisha
AU - Cate, Steven
AU - Osborn, Sean
AU - Mojsilovic, Aleksandar
AU - VanGundy, Rodney
AU - Bardet, Wilfried
AU - Duty, Andrew
AU - Mojsilovic, Danijela
AU - Jackson, Kenneth
AU - Stastny, Peter
AU - Briggs, David
AU - Zehnder, Daniel
AU - Higgins, Rob
AU - Hildebrand, William
N1 - Funding Information:
This work is supported by a Grant from Oklahoma’s Economic Development Generating Excellence ( EDGE10-026-FP ).
PY - 2014/3
Y1 - 2014/3
N2 - Immunizing events including pregnancy, transfusions, and transplantation promote strong alloantibody responses to HLA. Such alloantibodies to HLA preclude organ transplantation, foster hyperacute rejection, and contribute to chronic transplant failure. Diagnostic antibody-screening assays detect alloreactive antibodies, yet key attributes including antibody concentration and isotype remain largely unexplored. The goal here was to provide a detailed profile of allogeneic antibodies to class II HLA. Methodologically, alloantibodies were purified from sensitized patient sera using an HLA-DR11 immunoaffinity column and subsequently categorized. Antibodies to DR11 were found to fix complement, exist at a median serum concentration of 2.3. μg/mL, consist of all isotypes, and isotypes IgG2, IgM, and IgE were elevated. Because multimeric isotypes can confound diagnostic determinations of antibody concentration, IgM and IgA isotypes were removed and DR11-IgG tested alone. Despite removal of multimeric isotypes, patient-to-patient antibody concentrations did not correlate with MFI values. In conclusion, allogeneic antibody responses to DR11 are comprised of all antibody isotypes at differing proportions, these combined isotypes fix complement at nominal serum concentrations, and enhancements other than the removal of IgM and IgA multimeric isotypes may be required if MFI is to be used as a means of determining anti-HLA serum antibody concentrations in diagnostic clinical assays.
AB - Immunizing events including pregnancy, transfusions, and transplantation promote strong alloantibody responses to HLA. Such alloantibodies to HLA preclude organ transplantation, foster hyperacute rejection, and contribute to chronic transplant failure. Diagnostic antibody-screening assays detect alloreactive antibodies, yet key attributes including antibody concentration and isotype remain largely unexplored. The goal here was to provide a detailed profile of allogeneic antibodies to class II HLA. Methodologically, alloantibodies were purified from sensitized patient sera using an HLA-DR11 immunoaffinity column and subsequently categorized. Antibodies to DR11 were found to fix complement, exist at a median serum concentration of 2.3. μg/mL, consist of all isotypes, and isotypes IgG2, IgM, and IgE were elevated. Because multimeric isotypes can confound diagnostic determinations of antibody concentration, IgM and IgA isotypes were removed and DR11-IgG tested alone. Despite removal of multimeric isotypes, patient-to-patient antibody concentrations did not correlate with MFI values. In conclusion, allogeneic antibody responses to DR11 are comprised of all antibody isotypes at differing proportions, these combined isotypes fix complement at nominal serum concentrations, and enhancements other than the removal of IgM and IgA multimeric isotypes may be required if MFI is to be used as a means of determining anti-HLA serum antibody concentrations in diagnostic clinical assays.
UR - http://www.scopus.com/inward/record.url?scp=84893800075&partnerID=8YFLogxK
U2 - 10.1016/j.humimm.2013.11.015
DO - 10.1016/j.humimm.2013.11.015
M3 - Article
C2 - 24269696
AN - SCOPUS:84893800075
SN - 0198-8859
VL - 75
SP - 261
EP - 270
JO - Human Immunology
JF - Human Immunology
IS - 3
ER -