Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid

Fanyu Meng; Benjamin J. Cargile; Steven M. Patrie; Jeffrey R. Johnson; Thomas N. Corso; Shaun M. Mcloughlin; Neil L. Kelleher

Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid

Fanyu Meng, Benjamin J. Cargile, Steven M. Patrie, Jeffrey R. Johnson, Thomas N. Corso, Shaun M. Mcloughlin, Neil L. Kelleher

Research output: Contribution to conference › Paper › peer-review

Abstract

Analysis of yeast proteins for direct fragmentation in a quadrupole fourier transform mass spectrometry (FTMS) was discussed. Yeast ribosomal proteins with 100% sequence coverage were also analyzed to determine percent occupancy for known post-translational modifications (PTM). S. cerevisiae cells were grown to mid-log phase in YDP media and whole cell extract and ribosomal fractions were obtained following standard procedures. Results show that the method is capable of identifying proteins with 100% sequence coverage at both intact molecular and fragment levels.

Original language	English
Pages	49-50
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2/06/02 → 6/06/02

Cite this

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title = "Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid",

abstract = "Analysis of yeast proteins for direct fragmentation in a quadrupole fourier transform mass spectrometry (FTMS) was discussed. Yeast ribosomal proteins with 100% sequence coverage were also analyzed to determine percent occupancy for known post-translational modifications (PTM). S. cerevisiae cells were grown to mid-log phase in YDP media and whole cell extract and ribosomal fractions were obtained following standard procedures. Results show that the method is capable of identifying proteins with 100% sequence coverage at both intact molecular and fragment levels.",

author = "Fanyu Meng and Cargile, {Benjamin J.} and Patrie, {Steven M.} and Johnson, {Jeffrey R.} and Corso, {Thomas N.} and Mcloughlin, {Shaun M.} and Kelleher, {Neil L.}",

year = "2002",

language = "English",

pages = "49--50",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid

AU - Meng, Fanyu

AU - Cargile, Benjamin J.

AU - Patrie, Steven M.

AU - Johnson, Jeffrey R.

AU - Corso, Thomas N.

AU - Mcloughlin, Shaun M.

AU - Kelleher, Neil L.

PY - 2002

Y1 - 2002

N2 - Analysis of yeast proteins for direct fragmentation in a quadrupole fourier transform mass spectrometry (FTMS) was discussed. Yeast ribosomal proteins with 100% sequence coverage were also analyzed to determine percent occupancy for known post-translational modifications (PTM). S. cerevisiae cells were grown to mid-log phase in YDP media and whole cell extract and ribosomal fractions were obtained following standard procedures. Results show that the method is capable of identifying proteins with 100% sequence coverage at both intact molecular and fragment levels.

AB - Analysis of yeast proteins for direct fragmentation in a quadrupole fourier transform mass spectrometry (FTMS) was discussed. Yeast ribosomal proteins with 100% sequence coverage were also analyzed to determine percent occupancy for known post-translational modifications (PTM). S. cerevisiae cells were grown to mid-log phase in YDP media and whole cell extract and ribosomal fractions were obtained following standard procedures. Results show that the method is capable of identifying proteins with 100% sequence coverage at both intact molecular and fragment levels.

UR - http://www.scopus.com/inward/record.url?scp=2442685752&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442685752

SP - 49

EP - 50

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Processing complex mixtures of yeast proteins for direct fragmentation in a quadrupole-FTMS hybrid

Abstract

Conference

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