@article{d3feeb62fe6a4b29a842a6f92eb548de,
title = "Probing Neuropeptide Volume Transmission In Vivo by Simultaneous Near-Infrared Light-Triggered Release and Optical Sensing**",
abstract = "Neuropeptides are abundant signaling molecules in the central nervous system. Yet remarkably little is known about their spatiotemporal spread and biological activity. Here, we developed an integrated optical approach using Plasmonic nAnovesicles and cell-based neurotransmitter fluorescent engineered reporter (CNiFER), or PACE, to probe neuropeptide signaling in the mouse neocortex. Small volumes (fL to pL) of exogenously supplied somatostatin-14 (SST) can be rapidly released under near-infrared light stimulation from nanovesicles implanted in the brain and detected by SST2 CNiFERs with nM sensitivity. Our measurements reveal reduced but synchronized SST transmission within 130 μm, and markedly smaller and delayed transmission at longer distances. These measurements enabled a quantitative estimation of the SST loss rate due to peptide degradation and binding. PACE offers a new tool for determining the spatiotemporal scales of neuropeptide volume transmission and signaling in the brain.",
keywords = "Biosensors, Brain, Neuropeptide Release, Neuropeptide Transmission, Plasmonic Nanovesicles",
author = "Hejian Xiong and Emre Lacin and Hui Ouyang and Aditi Naik and Xueqi Xu and Chen Xie and Jonghae Youn and Wilson, {Blake A.} and Krutin Kumar and Tyler Kern and Erin Aisenberg and Daniel Kircher and Xiuying Li and Zasadzinski, {Joseph A.} and Celine Mateo and David Kleinfeld and Sabina Hrabetova and Slesinger, {Paul A.} and Zhenpeng Qin",
note = "Funding Information: We thank Dr. Oliver Griesbeck for providing the Twitch2B cDNA clone and discussions. Schematic figures were made with BioRender.com. This work was partially supported by National Science Foundation under award number 1631910 (Z.Q.), National Institute of Neurological Disorders and Stroke of the National Institutes of Health (NIH) under award number RF1NS110499 (Z.Q., P.A.S.), National Institute of Mental Health of NIH under award number R01MH111499 (P.A.S.), Human Frontier Science Program (HFSP) under award RGP0036/2020 (S.H.), and a postdoc research grant from the Phospholipid Research Center (Heidelberg, Germany) to H.X. Publisher Copyright: {\textcopyright} 2022 Wiley-VCH GmbH.",
year = "2022",
doi = "10.1002/anie.202206122",
language = "English",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley and Sons Ltd",
}