TY - JOUR
T1 - Probing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging
AU - Senders, Max L.
AU - Meerwaldt, Anu E.
AU - van Leent, Mandy M.T.
AU - Sanchez-Gaytan, Brenda L.
AU - van de Voort, Jan C.
AU - Toner, Yohana C.
AU - Maier, Alexander
AU - Klein, Emma D.
AU - Sullivan, Nathaniel A.T.
AU - Sofias, Alexandros Marios
AU - Groenen, Hannah
AU - Faries, Christopher
AU - Oosterwijk, Roderick S.
AU - van Leeuwen, Esther M.
AU - Fay, Francois
AU - Chepurko, Elena
AU - Reiner, Thomas
AU - Duivenvoorden, Raphael
AU - Zangi, Lior
AU - Dijkhuizen, Rick M.
AU - Hak, Sjoerd
AU - Swirski, Filip K.
AU - Nahrendorf, Matthias
AU - Pérez-Medina, Carlos
AU - Teunissen, Abraham J.P.
AU - Fayad, Zahi A.
AU - Calcagno, Claudia
AU - Strijkers, Gustav J.
AU - Mulder, Willem J.M.
N1 - Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Ischaemic heart disease evokes a complex immune response. However, tools to track the systemic behaviour and dynamics of leukocytes non-invasively in vivo are lacking. Here, we present a multimodal hot-spot imaging approach using an innovative high-density lipoprotein-derived nanotracer with a perfluoro-crown ether payload (19F-HDL) to allow myeloid cell tracking by 19F magnetic resonance imaging. The 19F-HDL nanotracer can additionally be labelled with zirconium-89 and fluorophores to detect myeloid cells by in vivo positron emission tomography imaging and optical modalities, respectively. Using our nanotracer in atherosclerotic mice with myocardial infarction, we observed rapid myeloid cell egress from the spleen and bone marrow by in vivo 19F-HDL magnetic resonance imaging. Concurrently, using ex vivo techniques, we showed that circulating pro-inflammatory myeloid cells accumulated in atherosclerotic plaques and at the myocardial infarct site. Our multimodality imaging approach is a valuable addition to the immunology toolbox, enabling the study of complex myeloid cell behaviour dynamically.
AB - Ischaemic heart disease evokes a complex immune response. However, tools to track the systemic behaviour and dynamics of leukocytes non-invasively in vivo are lacking. Here, we present a multimodal hot-spot imaging approach using an innovative high-density lipoprotein-derived nanotracer with a perfluoro-crown ether payload (19F-HDL) to allow myeloid cell tracking by 19F magnetic resonance imaging. The 19F-HDL nanotracer can additionally be labelled with zirconium-89 and fluorophores to detect myeloid cells by in vivo positron emission tomography imaging and optical modalities, respectively. Using our nanotracer in atherosclerotic mice with myocardial infarction, we observed rapid myeloid cell egress from the spleen and bone marrow by in vivo 19F-HDL magnetic resonance imaging. Concurrently, using ex vivo techniques, we showed that circulating pro-inflammatory myeloid cells accumulated in atherosclerotic plaques and at the myocardial infarct site. Our multimodality imaging approach is a valuable addition to the immunology toolbox, enabling the study of complex myeloid cell behaviour dynamically.
UR - http://www.scopus.com/inward/record.url?scp=85083718716&partnerID=8YFLogxK
U2 - 10.1038/s41565-020-0642-4
DO - 10.1038/s41565-020-0642-4
M3 - Article
C2 - 32313216
AN - SCOPUS:85083718716
SN - 1748-3387
VL - 15
SP - 398
EP - 405
JO - Nature Nanotechnology
JF - Nature Nanotechnology
IS - 5
ER -