TY - JOUR
T1 - Pro-α2(v) collagen gene; pairwise analysis of the amino-propeptide coding domain, and Cross-species comparison of the promoter sequence
AU - Truter, Sharada
AU - Andrikopoulos, Konstantinos
AU - Liberto, Maurizio Di
AU - Womack, Lawrence
AU - Ramirez, Francesco
N1 - Funding Information:
The authors wish to thank Ms. R. Lingeza for preparing the manuscript. This work was supported by a grant from The National Institutes of Health (AR-38648). This is article 112 from the Brookdale Center for Molecular Biology.
PY - 1993
Y1 - 1993
N2 - Type V collagen is a minor represented and poorly characterized fibrillar collagen type. Previous cDNA cloning experiments showed that the amino-propeptide of the pro-α2(V) chain shares structural features in common with pro-α1(I), proα1(II) and pro-α1(III) collagens. In the present paper, this analysis was extended to the gene level. Accordingly the exon/intron arrangement of the amino-propeptide coding domain was compared among pro-α1(I) (COL1A1), proα1(II) (COL2A1), pro-α1(III) (CO13A1) and pro-α2(V) (COL5A2) collagen genes. This revealed that COL3A1 is the most closely related gene to COL5A2. Based on the assumption that critical regulatory elements might be phylogenetically conserved, we also compared the promoter sequences of the mouse and human COL5A2 genes. This revealed the highest level of sequence homology (97% in a 52-bp segment which was previously shown to be essential in conferring cell type specificity to the human promoter.
AB - Type V collagen is a minor represented and poorly characterized fibrillar collagen type. Previous cDNA cloning experiments showed that the amino-propeptide of the pro-α2(V) chain shares structural features in common with pro-α1(I), proα1(II) and pro-α1(III) collagens. In the present paper, this analysis was extended to the gene level. Accordingly the exon/intron arrangement of the amino-propeptide coding domain was compared among pro-α1(I) (COL1A1), proα1(II) (COL2A1), pro-α1(III) (CO13A1) and pro-α2(V) (COL5A2) collagen genes. This revealed that COL3A1 is the most closely related gene to COL5A2. Based on the assumption that critical regulatory elements might be phylogenetically conserved, we also compared the promoter sequences of the mouse and human COL5A2 genes. This revealed the highest level of sequence homology (97% in a 52-bp segment which was previously shown to be essential in conferring cell type specificity to the human promoter.
KW - Collagen gene structure
KW - Collagen regulation
KW - Pro-α2(V) collagen
UR - http://www.scopus.com/inward/record.url?scp=0027361548&partnerID=8YFLogxK
U2 - 10.3109/03008209309061966
DO - 10.3109/03008209309061966
M3 - Article
C2 - 8339546
AN - SCOPUS:0027361548
SN - 0300-8207
VL - 29
SP - 51
EP - 59
JO - Connective Tissue Research
JF - Connective Tissue Research
IS - 1
ER -