@article{6bbb40de2a494b39a294a6838fd6e34a,
title = "Primary tumor associated macrophages activate programs of invasion and dormancy in disseminating tumor cells",
abstract = "Metastases are initiated by disseminated tumor cells (DTCs) that colonize distant organs. Growing evidence suggests that the microenvironment of the primary tumor primes DTCs for dormant or proliferative fates. However, the manner in which this occurs remains poorly understood. Here, using the Window for High-Resolution Intravital Imaging of the Lung (WHRIL), we study the live lung longitudinally and follow the fate of individual DTCs that spontaneously disseminate from orthotopic breast tumors. We find that spontaneously DTCs have increased levels of retention, increased speed of extravasation, and greater survival after extravasation, compared to experimentally metastasized tumor cells. Detailed analysis reveals that a subset of macrophages within the primary tumor induces a pro-dissemination and pro-dormancy DTC phenotype. Our work provides insight into how specific primary tumor microenvironments prime a subpopulation of cells for expression of proteins associated with dissemination and dormancy.",
author = "Lucia Borriello and Anouchka Coste and Brian Traub and Sharma, {Ved P.} and Karagiannis, {George S.} and Yu Lin and Yarong Wang and Xianjun Ye and Duran, {Camille L.} and Xiaoming Chen and Madeline Friedman and Sosa, {Maria Soledad} and Dan Sun and Erica Dalla and Singh, {Deepak K.} and Oktay, {Maja H.} and Aguirre-Ghiso, {Julio A.} and Condeelis, {John S.} and David Entenberg",
note = "Funding Information: The authors would like to thank Peng Guo, Vera DesMarais, and Hillary Guzik for assistance and support in the use of the Perkin Elmer 250 slide scanner and DeltaVision microscope. The authors would like to thank also Dr. Lalage Wakefield{\textquoteright}s lab at the NCI for the donation of the E0771 cell line. This work was supported by the NCI grants: R01-CA218024, S10-OD019961, P30-CA013330, F32-CA243350, CA237851, T32-CA200561, K12-GM102779, R01-CA109182, R01-CA218024, P30-CA196521, T32-CA078207, The NYS grant DOH01-ROWLEY-2019-00038; grants from METAvivor Career Development Award and HiberCell LLC; The Gruss-Lipper Biophotonics Center and its associated Integrated Imaging Program; and Jane A. and Myles P. Dempsey. JAA-G is a Samuel Waxman Cancer Research Foundation Investigator. Funding Information: The authors would like to thank Peng Guo, Vera DesMarais, and Hillary Guzik for assistance and support in the use of the Perkin Elmer 250 slide scanner and DeltaVision microscope. The authors would like to thank also Dr. Lalage Wakefield{\textquoteright}s lab at the NCI for the donation of the E0771 cell line. This work was supported by the NCI grants: R01-CA218024, S10-OD019961, P30-CA013330, F32-CA243350, CA237851, T32-CA200561, K12-GM102779, R01-CA109182, R01-CA218024, P30-CA196521, T32-CA078207, The NYS grant DOH01-ROWLEY-2019-00038; grants from METAvivor Career Development Award and HiberCell LLC; The Gruss-Lipper Biophotonics Center and its associated Integrated Imaging Program; and Jane A. and Myles P. Dempsey. JAA-G is a Samuel Waxman Cancer Research Foundation Investigator. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1038/s41467-022-28076-3",
language = "English",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}