TY - JOUR
T1 - Primary structure of the mouse sperm receptor polypeptide determined by genomic cloning
AU - Kinloch, R. A.
AU - Roller, R. J.
AU - Fimiani, C. M.
AU - Wassarman, D. A.
AU - Wassarman, P. M.
PY - 1988
Y1 - 1988
N2 - The mouse sperm receptor, a glycoprotein called ZP3, is synthesized and secreted by growing oocytes. It is present in more than a billion copies in the unfertilized egg's extracellular coat, or zona pellucida. We have cloned and characterized a region of the mouse (CD-1) genome that spans 10 kilobases of the ZP3 locus. The genomic clones described encompass the entire ZP3 coding region, which contains eight exons. The exons were identified, mapped, and sequenced, yielding the entire primary structure of the ZP3 polypeptide chain (424 amino acids; M(r), 46,300), which includes a 22-amino acid signal sequence. In addition, sequencing of genomic clones has revealed some unusual features of ZP3 mRNA and a region just downstream of the ZP3 gene.
AB - The mouse sperm receptor, a glycoprotein called ZP3, is synthesized and secreted by growing oocytes. It is present in more than a billion copies in the unfertilized egg's extracellular coat, or zona pellucida. We have cloned and characterized a region of the mouse (CD-1) genome that spans 10 kilobases of the ZP3 locus. The genomic clones described encompass the entire ZP3 coding region, which contains eight exons. The exons were identified, mapped, and sequenced, yielding the entire primary structure of the ZP3 polypeptide chain (424 amino acids; M(r), 46,300), which includes a 22-amino acid signal sequence. In addition, sequencing of genomic clones has revealed some unusual features of ZP3 mRNA and a region just downstream of the ZP3 gene.
UR - http://www.scopus.com/inward/record.url?scp=0023811049&partnerID=8YFLogxK
U2 - 10.1073/pnas.85.17.6409
DO - 10.1073/pnas.85.17.6409
M3 - Article
C2 - 2842770
AN - SCOPUS:0023811049
SN - 0027-8424
VL - 85
SP - 6409
EP - 6413
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17
ER -