Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel

Yoshihiro Kubo; Eitan Reuveny; Paul A. Slesinger; Yuh Nung Jan; Lily Y. Jan

doi:10.1038/364802a0

Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel

Yoshihiro Kubo, Eitan Reuveny, Paul A. Slesinger, Yuh Nung Jan, Lily Y. Jan

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580 Scopus citations

Abstract

PARASYMPATHETIC nerve stimulation causes slowing of the heart rate by activation of muscarinic receptors and the subsequent opening of muscarinic K⁺ channels in the sinoatrial node and atrium^1-4. This inwardly rectifying K⁺ channel is coupled directly with G protein^5-10. Based on sequence homology with cloned inwardly rectifying K⁺ channels, ROMK1 (ref. 11) and IRK1 (ref. 12), we have isolated a complementary DNA for a G-protein-coupled inwardly rectifying K ⁺ channel (GIRK1) from rat heart. The GIRK1 channel probably corresponds to the muscarinic K channel because (1) its functional properties resemble those of the a trial muscarinic K⁺ channel and (2) its messenger RNA is much more abundant in the atrium than in the ventricle. In addition, GIRK1 mRNA is expressed not only in the heart but also in the brain.

Original language	English
Pages (from-to)	802-806
Number of pages	5
Journal	Nature
Volume	364
Issue number	6440
DOIs	https://doi.org/10.1038/364802a0
State	Published - 1993
Externally published	Yes

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title = "Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel",

abstract = "PARASYMPATHETIC nerve stimulation causes slowing of the heart rate by activation of muscarinic receptors and the subsequent opening of muscarinic K+ channels in the sinoatrial node and atrium1-4. This inwardly rectifying K+ channel is coupled directly with G protein5-10. Based on sequence homology with cloned inwardly rectifying K+ channels, ROMK1 (ref. 11) and IRK1 (ref. 12), we have isolated a complementary DNA for a G-protein-coupled inwardly rectifying K + channel (GIRK1) from rat heart. The GIRK1 channel probably corresponds to the muscarinic K channel because (1) its functional properties resemble those of the a trial muscarinic K+ channel and (2) its messenger RNA is much more abundant in the atrium than in the ventricle. In addition, GIRK1 mRNA is expressed not only in the heart but also in the brain.",

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AU - Kubo, Yoshihiro

AU - Reuveny, Eitan

AU - Slesinger, Paul A.

AU - Jan, Yuh Nung

AU - Jan, Lily Y.

PY - 1993

Y1 - 1993

N2 - PARASYMPATHETIC nerve stimulation causes slowing of the heart rate by activation of muscarinic receptors and the subsequent opening of muscarinic K+ channels in the sinoatrial node and atrium1-4. This inwardly rectifying K+ channel is coupled directly with G protein5-10. Based on sequence homology with cloned inwardly rectifying K+ channels, ROMK1 (ref. 11) and IRK1 (ref. 12), we have isolated a complementary DNA for a G-protein-coupled inwardly rectifying K + channel (GIRK1) from rat heart. The GIRK1 channel probably corresponds to the muscarinic K channel because (1) its functional properties resemble those of the a trial muscarinic K+ channel and (2) its messenger RNA is much more abundant in the atrium than in the ventricle. In addition, GIRK1 mRNA is expressed not only in the heart but also in the brain.

AB - PARASYMPATHETIC nerve stimulation causes slowing of the heart rate by activation of muscarinic receptors and the subsequent opening of muscarinic K+ channels in the sinoatrial node and atrium1-4. This inwardly rectifying K+ channel is coupled directly with G protein5-10. Based on sequence homology with cloned inwardly rectifying K+ channels, ROMK1 (ref. 11) and IRK1 (ref. 12), we have isolated a complementary DNA for a G-protein-coupled inwardly rectifying K + channel (GIRK1) from rat heart. The GIRK1 channel probably corresponds to the muscarinic K channel because (1) its functional properties resemble those of the a trial muscarinic K+ channel and (2) its messenger RNA is much more abundant in the atrium than in the ventricle. In addition, GIRK1 mRNA is expressed not only in the heart but also in the brain.

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Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel

Abstract

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