TY - JOUR
T1 - Pretreatment combination reduces remote organ damage secondary to intestinal reperfusion injury in mice
T2 - Follow-up study
AU - Stringa, P.
AU - Lausada, N.
AU - Romanin, D.
AU - Portiansky, E.
AU - Zanuzzi, C.
AU - Machuca, M.
AU - Gondolesi, G.
AU - Rumbo, M.
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Background Intestinal ischemia-reperfusion injury occurs after different surgical treatments, including intestinal transplantation. This harmful process may have an effect in remote organs, leading to multiple organ dysfunction syndrome and death. Therefore, to establish strategies to attenuate local and remote damage constitutes a challenge for experimental and clinical surgeons in the intestinal surgical field. Methods We evaluated the effect of ischemic preconditioning and tacrolimus pretreatment applied alone and in combination against local and remote damage caused by prolonged intestinal ischemia-reperfusion injury in a mouse model of warm ischemia. Results Ischemic preconditioning applied alone and in combination with tacrolimus decreased histological damage (P <.05), number of apoptotic cells (P <.05), nitrosative stress (P <.01), and serum lactate dehydrogenase activity (P <.05) and lowered uremia (P <.05) compared with untreated post-reperfused intestines. Regarding remote organ damage, combination therapy was the unique condition able to attenuate lung (mainly neutrophil infiltration and hemorrhage), liver (sinusoidal congestion and hepatic vacuolization), and kidney (acute tubular necrosis and hydropic degeneration) histological alterations (P <.05), compared with the untreated group. Conclusions These results support the application of these strategies in combination to minimize the impact of ischemia-reperfusion injury in the whole organism as a strategy to prevent multiple organ dysfunction syndromes and minimize the clinical impact.
AB - Background Intestinal ischemia-reperfusion injury occurs after different surgical treatments, including intestinal transplantation. This harmful process may have an effect in remote organs, leading to multiple organ dysfunction syndrome and death. Therefore, to establish strategies to attenuate local and remote damage constitutes a challenge for experimental and clinical surgeons in the intestinal surgical field. Methods We evaluated the effect of ischemic preconditioning and tacrolimus pretreatment applied alone and in combination against local and remote damage caused by prolonged intestinal ischemia-reperfusion injury in a mouse model of warm ischemia. Results Ischemic preconditioning applied alone and in combination with tacrolimus decreased histological damage (P <.05), number of apoptotic cells (P <.05), nitrosative stress (P <.01), and serum lactate dehydrogenase activity (P <.05) and lowered uremia (P <.05) compared with untreated post-reperfused intestines. Regarding remote organ damage, combination therapy was the unique condition able to attenuate lung (mainly neutrophil infiltration and hemorrhage), liver (sinusoidal congestion and hepatic vacuolization), and kidney (acute tubular necrosis and hydropic degeneration) histological alterations (P <.05), compared with the untreated group. Conclusions These results support the application of these strategies in combination to minimize the impact of ischemia-reperfusion injury in the whole organism as a strategy to prevent multiple organ dysfunction syndromes and minimize the clinical impact.
UR - http://www.scopus.com/inward/record.url?scp=84959152796&partnerID=8YFLogxK
U2 - 10.1016/j.transproceed.2015.12.002
DO - 10.1016/j.transproceed.2015.12.002
M3 - Article
C2 - 26915870
AN - SCOPUS:84959152796
SN - 0041-1345
VL - 48
SP - 210
EP - 216
JO - Transplantation Proceedings
JF - Transplantation Proceedings
IS - 1
ER -