Presenilin 1 mutations linked to familial Alzheimer's disease increase the intracellular levels of amyloid β-protein 1-42 and its N-terminally truncated variant(s) which are generated at distinct sites

Shinji Sudoh, Yuuki Kawamura, Rong Wang, Takaomi C. Saido, Fumitaka Oyama, Yoshiyuki Sakaki, Hiroto Komano, Katsuhiko Yanagisawa

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Mutations in the presenilin genes PS1 and PS2 cause the most common form of early-onset familial Alzheimer's disease. The influence of PS1 mutations on the generation of endogenous intracellular amyloid β-protein (Aβ) species was assessed using a highly sensitive immunoblotting technique with inducible mouse neuroblastoma (Neuro 2a) cell lines expressing the human wild-type (wt) or mutated PS1 (M146L or Δexon 10). The induction of mutated PS1 increased the intracellular levels of two distinct Aβ species ending at residue 42 that were likely to be Aβ1-42 and its N-terminally truncated variant(s) Aβx-42. The induction of mutated PS1 resulted in a higher level of intracellular Aβ1-42 than of intracellular Aβx-42, whereas extracellular levels of Aβ1-42 and Aβx-42 were increased proportionally. In addition, the intracellular generation of these Aβ42 species in wt and mutated PS1- induced cells was completely blocked by brefeldin A, whereas it exhibited differential sensitivities to monensin: the increased accumulation of intracellular Aβx-42 versus inhibition of intracellular Aβ1-42 generation. These data strongly suggest that Aβx-42 is generated n a proximal Golgi, whereas Aβ1-42 is generated in a distal Golgi and/or a post-Golgi compartment. Thus, it appears that PS1 mutations enhance the degree of 42- specific γ-secretase cleavage that occurs in the normal β-amyloid precursor protein processing pathway (a) in the endoplasmic reticulum or the early Golgi apparatus prior to β-secretase cleavage or (b) in the distinct sites where Aβx-42 and Aβ1-42 are generated.

Original languageEnglish
Pages (from-to)1535-1543
Number of pages9
JournalJournal of Neurochemistry
Volume71
Issue number4
DOIs
StatePublished - Oct 1998
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Amyloid β-protein
  • Presenilin 1

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