Prepubertal acrylamide exposure causes dose-response decreases in spermatic production and functionality with modulation of genes involved in the spermatogenesis in rats

Fernanda Ivanski, Viviane Matoso de Oliveira, Isabela Medeiros de Oliveira, Anderson Tadeu de Araújo Ramos, Selma Thaisa de Oliveira Tonete, Gabriel de Oliveira Hykavei, Paula Bargi-Souza, Dalton Luiz Schiessel, Anderson Joel Martino-Andrade, Marco Aurelio Romano, Renata Marino Romano

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The increase in human infertility prevalence due to male reproductive disorders has been associated with extensive endocrine-disrupting chemical (EDC) exposure. Acrylamide (AA) is a compound formed spontaneously during heat processing of some foods that are mainly consumed by children and adolescents. In this study, we evaluated the prepubertal AA exposure effects on male adult reproductive physiology using a prepubertal experimental model to analyze the pubertal development, spermatogenesis hormones levels and genes expression involved in male reproductive function. This study is the first one to use the validated protocol to correlate the AA exposure with puberty development, as well as the AA-induced endocrine disrupting effects on reproductive axis. AA did not affect the age at puberty, the reproductive organ's weight and serum hormonal levels. AA reduces spermatogenesis, induces morphological and functional defects on sperm and alters transcript expression of sexual hormone receptors (Ar and Esr2), the transcript expression of Tnf, Egr2, Rhcg and Lrrc34. These findings suggest that excessive AA consumption may impair their reproductive capacity at adulthood, despite no changes in hormonal profile being observed.

Original languageEnglish
Article number152428
JournalToxicology
Volume436
DOIs
StatePublished - 30 Apr 2020
Externally publishedYes

Keywords

  • Acrylamide
  • Developmental origin of health and disease (DOHaD)
  • Endocrine-disrupting chemical
  • Male reproductive system
  • Spermatogenesis
  • Toxicology

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