Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

Liyan Li; Xiaolu Jiang; Huashi Guan; Peng Wang

doi:10.1016/j.carres.2011.01.023

Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

Liyan Li, Xiaolu Jiang, Huashi Guan, Peng Wang

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Alginate lyase which was purified from the fermentation solution of marine bacteria Pseudomonas sp. HJZ216 was applied to hydrolyze algae alginate. Six oligosaccharides, including di- and trisaccharides, were isolated and purified through anion exchange chromatography. The oligosaccharide structures were elucidated based on electrospray ionization-mass spectrometry (ESI-MS) and 2D NMR spectra analysis.

Original language	English
Pages (from-to)	794-800
Number of pages	7
Journal	Carbohydrate Research
Volume	346
Issue number	6
DOIs	https://doi.org/10.1016/j.carres.2011.01.023
State	Published - 1 May 2011
Externally published	Yes

Keywords

Alginate
Alginate lyase
NMR
Oligosaccharide

Access to Document

10.1016/j.carres.2011.01.023

Cite this

@article{a3e84454093d497f9296c934f74502fa,

title = "Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216",

abstract = "Alginate lyase which was purified from the fermentation solution of marine bacteria Pseudomonas sp. HJZ216 was applied to hydrolyze algae alginate. Six oligosaccharides, including di- and trisaccharides, were isolated and purified through anion exchange chromatography. The oligosaccharide structures were elucidated based on electrospray ionization-mass spectrometry (ESI-MS) and 2D NMR spectra analysis.",

keywords = "Alginate, Alginate lyase, NMR, Oligosaccharide",

author = "Liyan Li and Xiaolu Jiang and Huashi Guan and Peng Wang",

note = "Funding Information: The work was supported partly by grant from {\textquoteleft}The Shandong Province Key Project of China{\textquoteright} (Project No. 2007ZHZX11204) and in part by National Natural Science Foundation of China (Grant No. 30901122 ).",

year = "2011",

month = may,

day = "1",

doi = "10.1016/j.carres.2011.01.023",

language = "English",

volume = "346",

pages = "794--800",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier Ltd.",

number = "6",

}

TY - JOUR

T1 - Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

AU - Li, Liyan

AU - Jiang, Xiaolu

AU - Guan, Huashi

AU - Wang, Peng

N1 - Funding Information: The work was supported partly by grant from ‘The Shandong Province Key Project of China’ (Project No. 2007ZHZX11204) and in part by National Natural Science Foundation of China (Grant No. 30901122 ).

PY - 2011/5/1

Y1 - 2011/5/1

N2 - Alginate lyase which was purified from the fermentation solution of marine bacteria Pseudomonas sp. HJZ216 was applied to hydrolyze algae alginate. Six oligosaccharides, including di- and trisaccharides, were isolated and purified through anion exchange chromatography. The oligosaccharide structures were elucidated based on electrospray ionization-mass spectrometry (ESI-MS) and 2D NMR spectra analysis.

AB - Alginate lyase which was purified from the fermentation solution of marine bacteria Pseudomonas sp. HJZ216 was applied to hydrolyze algae alginate. Six oligosaccharides, including di- and trisaccharides, were isolated and purified through anion exchange chromatography. The oligosaccharide structures were elucidated based on electrospray ionization-mass spectrometry (ESI-MS) and 2D NMR spectra analysis.

KW - Alginate

KW - Alginate lyase

KW - NMR

KW - Oligosaccharide

UR - http://www.scopus.com/inward/record.url?scp=79953315946&partnerID=8YFLogxK

U2 - 10.1016/j.carres.2011.01.023

DO - 10.1016/j.carres.2011.01.023

M3 - Article

C2 - 21371694

AN - SCOPUS:79953315946

SN - 0008-6215

VL - 346

SP - 794

EP - 800

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 6

ER -

Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

Abstract

Keywords

Access to Document

Fingerprint

Cite this