Preparation of a high-active microsomal monooxygenase system reconstituted by means of self-assembly

Ilya B. Tsyrlov; Vladimir M. Mishin; Olga A. Gromova; Nataliya E. Zakharova; Nyacheslav V. lyakhovich

doi:10.1016/0006-2952(77)90017-X

Preparation of a high-active microsomal monooxygenase system reconstituted by means of self-assembly

Ilya B. Tsyrlov
, Vladimir M. Mishin
, Olga A. Gromova
, Nataliya E. Zakharova
, Nyacheslav V. lyakhovich

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The present work is devoted to study of the role of glycerol in preservation of the lipoprotein nature of cytochrome P-450 during the stages of sodium cholate-caused solubilization of microsomal fraction and subsequent self-assembly of a monooxygenase system after prolonged dialysis. The system thus reconstituted is characterized by the highest catalytic activity of cytochrome P-450 compared to other reconstituted liver microsomal hydroxylating systems presently described in literature.

Original language	English
Pages (from-to)	2061-2063
Number of pages	3
Journal	Biochemical Pharmacology
Volume	26
Issue number	21
DOIs	https://doi.org/10.1016/0006-2952(77)90017-X
State	Published - 1 Nov 1977
Externally published	Yes

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10.1016/0006-2952(77)90017-X

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title = "Preparation of a high-active microsomal monooxygenase system reconstituted by means of self-assembly",

abstract = "The present work is devoted to study of the role of glycerol in preservation of the lipoprotein nature of cytochrome P-450 during the stages of sodium cholate-caused solubilization of microsomal fraction and subsequent self-assembly of a monooxygenase system after prolonged dialysis. The system thus reconstituted is characterized by the highest catalytic activity of cytochrome P-450 compared to other reconstituted liver microsomal hydroxylating systems presently described in literature.",

author = "Tsyrlov, \{Ilya B.\} and Mishin, \{Vladimir M.\} and Gromova, \{Olga A.\} and Zakharova, \{Nataliya E.\} and lyakhovich, \{Nyacheslav V.\}",

year = "1977",

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doi = "10.1016/0006-2952(77)90017-X",

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T1 - Preparation of a high-active microsomal monooxygenase system reconstituted by means of self-assembly

AU - Tsyrlov, Ilya B.

AU - Mishin, Vladimir M.

AU - Gromova, Olga A.

AU - Zakharova, Nataliya E.

AU - lyakhovich, Nyacheslav V.

PY - 1977/11/1

Y1 - 1977/11/1

N2 - The present work is devoted to study of the role of glycerol in preservation of the lipoprotein nature of cytochrome P-450 during the stages of sodium cholate-caused solubilization of microsomal fraction and subsequent self-assembly of a monooxygenase system after prolonged dialysis. The system thus reconstituted is characterized by the highest catalytic activity of cytochrome P-450 compared to other reconstituted liver microsomal hydroxylating systems presently described in literature.

AB - The present work is devoted to study of the role of glycerol in preservation of the lipoprotein nature of cytochrome P-450 during the stages of sodium cholate-caused solubilization of microsomal fraction and subsequent self-assembly of a monooxygenase system after prolonged dialysis. The system thus reconstituted is characterized by the highest catalytic activity of cytochrome P-450 compared to other reconstituted liver microsomal hydroxylating systems presently described in literature.

UR - https://www.scopus.com/pages/publications/0017639922

U2 - 10.1016/0006-2952(77)90017-X

DO - 10.1016/0006-2952(77)90017-X

M3 - Article

C2 - 921816

AN - SCOPUS:0017639922

SN - 0006-2952

VL - 26

SP - 2061

EP - 2063

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 21

ER -

Preparation of a high-active microsomal monooxygenase system reconstituted by means of self-assembly

Abstract

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