Prenatal protein malnutrition decreases KCNJ3 and 2DG activity in rat prefrontal cortex

A. C. Amaral; M. Jakovcevski; J. A. McGaughy; S. K. Calderwood; D. J. Mokler; R. J. Rushmore; J. R. Galler; S. A. Akbarian; D. L. Rosene

doi:10.1016/j.neuroscience.2014.11.005

Prenatal protein malnutrition decreases KCNJ3 and 2DG activity in rat prefrontal cortex

A. C. Amaral, M. Jakovcevski, J. A. McGaughy, S. K. Calderwood, D. J. Mokler, R. J. Rushmore, J. R. Galler, S. A. Akbarian, D. L. Rosene

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10 Scopus citations

Abstract

Prenatal protein malnutrition (PPM) in rats causes enduring changes in brain and behavior including increased cognitive rigidity and decreased inhibitory control. A preliminary gene microarray screen of PPM rat prefrontal cortex (PFC) identified alterations in KCNJ3 (GIRK1/Kir3.1), a gene important for regulating neuronal excitability. Follow-up with polymerase chain reaction and Western blot showed decreased KCNJ3 expression in the PFC, but not hippocampus or brainstem. To verify localization of the effect to the PFC, baseline regional brain activity was assessed with ¹⁴C-2-deoxyglucose. Results showed decreased activation in the PFC but not hippocampus. Together these findings point to the unique vulnerability of the PFC to the nutritional insult during early brain development, with enduring effects in adulthood on KCNJ3 expression and baseline metabolic activity.

Original language	English
Pages (from-to)	79-86
Number of pages	8
Journal	Neuroscience
Volume	286
DOIs	https://doi.org/10.1016/j.neuroscience.2014.11.005
State	Published - 2 Feb 2015

Keywords

2-deoxyglucose
Brain activity
GIRK1
KCNJ3
Prefrontal cortex
Prenatal protein malnutrition

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10.1016/j.neuroscience.2014.11.005

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title = "Prenatal protein malnutrition decreases KCNJ3 and 2DG activity in rat prefrontal cortex",

abstract = "Prenatal protein malnutrition (PPM) in rats causes enduring changes in brain and behavior including increased cognitive rigidity and decreased inhibitory control. A preliminary gene microarray screen of PPM rat prefrontal cortex (PFC) identified alterations in KCNJ3 (GIRK1/Kir3.1), a gene important for regulating neuronal excitability. Follow-up with polymerase chain reaction and Western blot showed decreased KCNJ3 expression in the PFC, but not hippocampus or brainstem. To verify localization of the effect to the PFC, baseline regional brain activity was assessed with 14C-2-deoxyglucose. Results showed decreased activation in the PFC but not hippocampus. Together these findings point to the unique vulnerability of the PFC to the nutritional insult during early brain development, with enduring effects in adulthood on KCNJ3 expression and baseline metabolic activity.",

keywords = "2-deoxyglucose, Brain activity, GIRK1, KCNJ3, Prefrontal cortex, Prenatal protein malnutrition",

author = "Amaral, {A. C.} and M. Jakovcevski and McGaughy, {J. A.} and Calderwood, {S. K.} and Mokler, {D. J.} and Rushmore, {R. J.} and Galler, {J. R.} and Akbarian, {S. A.} and Rosene, {D. L.}",

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year = "2015",

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doi = "10.1016/j.neuroscience.2014.11.005",

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AU - Amaral, A. C.

AU - Jakovcevski, M.

AU - McGaughy, J. A.

AU - Calderwood, S. K.

AU - Mokler, D. J.

AU - Rushmore, R. J.

AU - Galler, J. R.

AU - Akbarian, S. A.

AU - Rosene, D. L.

PY - 2015/2/2

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N2 - Prenatal protein malnutrition (PPM) in rats causes enduring changes in brain and behavior including increased cognitive rigidity and decreased inhibitory control. A preliminary gene microarray screen of PPM rat prefrontal cortex (PFC) identified alterations in KCNJ3 (GIRK1/Kir3.1), a gene important for regulating neuronal excitability. Follow-up with polymerase chain reaction and Western blot showed decreased KCNJ3 expression in the PFC, but not hippocampus or brainstem. To verify localization of the effect to the PFC, baseline regional brain activity was assessed with 14C-2-deoxyglucose. Results showed decreased activation in the PFC but not hippocampus. Together these findings point to the unique vulnerability of the PFC to the nutritional insult during early brain development, with enduring effects in adulthood on KCNJ3 expression and baseline metabolic activity.

AB - Prenatal protein malnutrition (PPM) in rats causes enduring changes in brain and behavior including increased cognitive rigidity and decreased inhibitory control. A preliminary gene microarray screen of PPM rat prefrontal cortex (PFC) identified alterations in KCNJ3 (GIRK1/Kir3.1), a gene important for regulating neuronal excitability. Follow-up with polymerase chain reaction and Western blot showed decreased KCNJ3 expression in the PFC, but not hippocampus or brainstem. To verify localization of the effect to the PFC, baseline regional brain activity was assessed with 14C-2-deoxyglucose. Results showed decreased activation in the PFC but not hippocampus. Together these findings point to the unique vulnerability of the PFC to the nutritional insult during early brain development, with enduring effects in adulthood on KCNJ3 expression and baseline metabolic activity.

KW - 2-deoxyglucose

KW - Brain activity

KW - GIRK1

KW - KCNJ3

KW - Prefrontal cortex

KW - Prenatal protein malnutrition

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EP - 86

JO - Neuroscience

JF - Neuroscience

ER -

Prenatal protein malnutrition decreases KCNJ3 and 2DG activity in rat prefrontal cortex

Abstract

Keywords

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