Preliminary study on the cleavage of fusion protein GST-CMIV with palladium(II) complex

Fei Dou; Feng Qiao; Jianzhong Hu; Tongyang Zhu; Xianxiu Xu; Dexue Zhu; Longgen Zhu

doi:10.1080/10826060008544946

Preliminary study on the cleavage of fusion protein GST-CMIV with palladium(II) complex

Fei Dou, Feng Qiao, Jianzhong Hu, Tongyang Zhu, Xianxiu Xu, Dexue Zhu, Longgen Zhu

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A novel method for post-treatment of gene-engineered proteins is reported. A coden of Cys-His unit is introduced into the N-terminal of cecropin CMIV by using PCR. The gene is expressed in E. coli fused with GST. After purification, the fusion protein is cleaved by [Pd(en)(H₂O)₂]²⁺ at the His-Arg bond and the cecropin CMIV with antibacterial activity is obtained. The preliminary results held some promise of success for application of the palladium(II) complex as cleavage agent for the production of peptide drugs from gene-engineering fusion proteins.

Original language	English
Pages (from-to)	69-78
Number of pages	10
Journal	Preparative Biochemistry and Biotechnology
Volume	30
Issue number	1
DOIs	https://doi.org/10.1080/10826060008544946
State	Published - 2000
Externally published	Yes

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title = "Preliminary study on the cleavage of fusion protein GST-CMIV with palladium(II) complex",

abstract = "A novel method for post-treatment of gene-engineered proteins is reported. A coden of Cys-His unit is introduced into the N-terminal of cecropin CMIV by using PCR. The gene is expressed in E. coli fused with GST. After purification, the fusion protein is cleaved by [Pd(en)(H2O)2]2+ at the His-Arg bond and the cecropin CMIV with antibacterial activity is obtained. The preliminary results held some promise of success for application of the palladium(II) complex as cleavage agent for the production of peptide drugs from gene-engineering fusion proteins.",

author = "Fei Dou and Feng Qiao and Jianzhong Hu and Tongyang Zhu and Xianxiu Xu and Dexue Zhu and Longgen Zhu",

note = "Funding Information: We thank the National Natural Science Foundation of China (#29871017) and the Natural Science Foundation of Jiangsu Province (#BK97015) for financial support.",

year = "2000",

doi = "10.1080/10826060008544946",

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journal = "Preparative Biochemistry and Biotechnology",

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TY - JOUR

T1 - Preliminary study on the cleavage of fusion protein GST-CMIV with palladium(II) complex

AU - Dou, Fei

AU - Qiao, Feng

AU - Hu, Jianzhong

AU - Zhu, Tongyang

AU - Xu, Xianxiu

AU - Zhu, Dexue

AU - Zhu, Longgen

N1 - Funding Information: We thank the National Natural Science Foundation of China (#29871017) and the Natural Science Foundation of Jiangsu Province (#BK97015) for financial support.

PY - 2000

Y1 - 2000

N2 - A novel method for post-treatment of gene-engineered proteins is reported. A coden of Cys-His unit is introduced into the N-terminal of cecropin CMIV by using PCR. The gene is expressed in E. coli fused with GST. After purification, the fusion protein is cleaved by [Pd(en)(H2O)2]2+ at the His-Arg bond and the cecropin CMIV with antibacterial activity is obtained. The preliminary results held some promise of success for application of the palladium(II) complex as cleavage agent for the production of peptide drugs from gene-engineering fusion proteins.

AB - A novel method for post-treatment of gene-engineered proteins is reported. A coden of Cys-His unit is introduced into the N-terminal of cecropin CMIV by using PCR. The gene is expressed in E. coli fused with GST. After purification, the fusion protein is cleaved by [Pd(en)(H2O)2]2+ at the His-Arg bond and the cecropin CMIV with antibacterial activity is obtained. The preliminary results held some promise of success for application of the palladium(II) complex as cleavage agent for the production of peptide drugs from gene-engineering fusion proteins.

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DO - 10.1080/10826060008544946

M3 - Article

C2 - 10701454

AN - SCOPUS:0034143469

SN - 1082-6068

VL - 30

SP - 69

EP - 78

JO - Preparative Biochemistry and Biotechnology

JF - Preparative Biochemistry and Biotechnology

IS - 1

ER -

Preliminary study on the cleavage of fusion protein GST-CMIV with palladium(II) complex

Abstract

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