@article{cefda314ce2940d49b6c9470c11571a6,
title = "Preimplantation embryo sexing by polymerase chain reaction amplification of the sry gene on single mouse blastomeres",
abstract = "Accurate and rapid sex determination of preimplantation embryos has great potential both in animal breeding and in human pathology. In the past, sex determination has been accomplished by cytogenetic or immunologic means and by polymerase chain reaction amplification of Y-chromosome-specific repetitive sequences. More recently, amplification of the Y-specific single-copy ZFY gene has been used in humans for sex determination of preimplantation embryos. The experiments reported here indicate that another Y-chromosome-specific single-copy gene, the sex-determining region gene (sry) can be successfully amplified from single mouse blastomeres. Blastocysts positive for sry amplification were reimplanted to foster mothers, and six of six newborns were male. We conclude that sry gene amplification can represent a good marker for embryo sex determination.",
author = "Mulder, \{Lubbertus C.\} and Sacco, \{Maria G.\} and Laura Mangiarini and John Brown and Angelo Collotta and Anna Villa and \{De Giovanni\}, \{Anna M.\} and Paolo Vezzoni and Libero Clerici",
note = "Funding Information: We are grateful to Dr. Mara Rossini for critically reading the manuscript, and to Miss Sophie Bevan for its careful preparation. This work was supported by grants from CNR, P.F. In-gegneria Genetica (to P.V.), P.F. ACRO (to A.V.), and AIRC (to A.V.). The technical assistance of D. Strina is acknowledged. Funding Information: From the Joint Research Center (L.C.M., A.C., L.C.), Environment Institute-Life Sciences, Center of European Communities, Ispra (Varese); the Institute of Advanced Biomedical Research (M.G.S., L.M., J.B., A.V., P.V.), National Council of Research, Milan; and the Institute of General Animal Technology (A.M. De G.), University of Milan, Milan, Italy. Address correspondence to Dr. L. Clerici, Joint Research Center, European Community, Ispra (VA), Italy. Received 9 November 1993; accepted 3 December 1993.",
year = "1993",
doi = "10.1016/1050-3862(93)90010-G",
language = "English",
volume = "10",
pages = "147--149",
journal = "New Biotechnology",
issn = "1050-3862",
publisher = "Elsevier B.V.",
number = "6",
}