TY - JOUR
T1 - Predicting disease course in ulcerative colitis using stool proteins identified through an aptamer-based screen
AU - Soomro, Sanam
AU - Venkateswaran, Suresh
AU - Vanarsa, Kamala
AU - Kharboutli, Marwa
AU - Nidhi, Malavika
AU - Susarla, Ramya
AU - Zhang, Ting
AU - Sasidharan, Prashanth
AU - Lee, Kyung Hyun
AU - Rosh, Joel
AU - Markowitz, James
AU - Pedroza, Claudia
AU - Denson, Lee A.
AU - Hyams, Jeffrey
AU - Kugathasan, Subra
AU - Mohan, Chandra
N1 - Funding Information:
We thank Anne Dodd, and Jarod Prince for sample processing, obtaining clinical metadata, and providing helpful comments to the manuscript. We would like to acknowledge the research coordinators at the study sites for their tireless attention, and the patients and families who agreed to participate in this important study. This work was supported by a research initiative grant from the Senior Research Award from Crohn’s and Colitis Foundation, New York, NY, under grant number 568731. This study was also supported by NIDDK grant numbers DK087696, and 5U01DK095745.
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12/1
Y1 - 2021/12/1
N2 - In the search for improved stool biomarkers for inflammatory bowel disease (IBD), an aptamer-based screen of 1129 stool proteins was conducted using stool samples from an IBD cohort. Here we report that of the 20 proteins subsequently validated by ELISA, stool Ferritin, Fibrinogen, Haptoglobin, Hemoglobin, Lipocalin-2, MMP-12, MMP-9, Myeloperoxidase, PGRP-S, Properdin, Resistin, Serpin A4, and TIMP-1 are significantly elevated in both ulcerative colitis (UC) and Crohn’s disease (CD) compared to controls. When tested in a longitudinal cohort of 50 UC patients at 4 time-points, fecal Fibrinogen, MMP-8, PGRP-S, and TIMP-2 show the strongest positive correlation with concurrent PUCAI and PGA scores and are superior to fecal calprotectin. Unlike fecal calprotectin, baseline stool Fibrinogen, MMP-12, PGRP-S, TIMP-1, and TIMP-2 can predict clinical remission at Week-4. Here we show that stool proteins identified using the comprehensive aptamer-based screen are superior to fecal calprotectin alone in disease monitoring and prediction in IBD.
AB - In the search for improved stool biomarkers for inflammatory bowel disease (IBD), an aptamer-based screen of 1129 stool proteins was conducted using stool samples from an IBD cohort. Here we report that of the 20 proteins subsequently validated by ELISA, stool Ferritin, Fibrinogen, Haptoglobin, Hemoglobin, Lipocalin-2, MMP-12, MMP-9, Myeloperoxidase, PGRP-S, Properdin, Resistin, Serpin A4, and TIMP-1 are significantly elevated in both ulcerative colitis (UC) and Crohn’s disease (CD) compared to controls. When tested in a longitudinal cohort of 50 UC patients at 4 time-points, fecal Fibrinogen, MMP-8, PGRP-S, and TIMP-2 show the strongest positive correlation with concurrent PUCAI and PGA scores and are superior to fecal calprotectin. Unlike fecal calprotectin, baseline stool Fibrinogen, MMP-12, PGRP-S, TIMP-1, and TIMP-2 can predict clinical remission at Week-4. Here we show that stool proteins identified using the comprehensive aptamer-based screen are superior to fecal calprotectin alone in disease monitoring and prediction in IBD.
UR - http://www.scopus.com/inward/record.url?scp=85109655611&partnerID=8YFLogxK
U2 - 10.1038/s41467-021-24235-0
DO - 10.1038/s41467-021-24235-0
M3 - Article
C2 - 34183667
AN - SCOPUS:85109655611
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 3989
ER -