Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies

Carolina Cubillos-Zapata; Iván Angulo; Horacio Almanza; Belén Borrego; María Zamora-Ceballos; José R. Castón; Ignacio Mena; Esther Blanco; Juan Bárcena

doi:10.1186/s13567-020-00785-x

Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies

Carolina Cubillos-Zapata, Iván Angulo, Horacio Almanza, Belén Borrego, María Zamora-Ceballos, José R. Castón, Ignacio Mena, Esther Blanco, Juan Bárcena

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We report the generation, characterization and epitope mapping of a panel of 26 monoclonal antibodies (MAbs) against the VP1 capsid protein of feline calicivirus (FCV). Two close but distinct linear epitopes were identified at the capsid outermost surface (P2 subdomain) of VP1, within the E5′HVR antigenic hypervariable region: one spanning amino acids 431-435 (PAGDY), highly conserved and recognized by non-neutralizing MAbs; and a second epitope spanning amino acids 445-451 (ITTANQY), highly variable and recognized by neutralizing MAbs. These antibodies might be valuable for diagnostic applications, as well as for further research in different aspects of the biology of FCV.

Original language	English
Article number	59
Journal	Veterinary Research
Volume	51
Issue number	1
DOIs	https://doi.org/10.1186/s13567-020-00785-x
State	Published - 1 May 2020

Access to Document

10.1186/s13567-020-00785-x

Cite this

Cubillos-Zapata, C., Angulo, I., Almanza, H., Borrego, B., Zamora-Ceballos, M., Castón, J. R., Mena, I., Blanco, E., & Bárcena, J. (2020). Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies. Veterinary Research, 51(1), Article 59. https://doi.org/10.1186/s13567-020-00785-x

@article{4b1d71acc62c4edaa7541bbece7c0f1e,

title = "Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies",

abstract = "We report the generation, characterization and epitope mapping of a panel of 26 monoclonal antibodies (MAbs) against the VP1 capsid protein of feline calicivirus (FCV). Two close but distinct linear epitopes were identified at the capsid outermost surface (P2 subdomain) of VP1, within the E5′HVR antigenic hypervariable region: one spanning amino acids 431-435 (PAGDY), highly conserved and recognized by non-neutralizing MAbs; and a second epitope spanning amino acids 445-451 (ITTANQY), highly variable and recognized by neutralizing MAbs. These antibodies might be valuable for diagnostic applications, as well as for further research in different aspects of the biology of FCV.",

author = "Carolina Cubillos-Zapata and Iv{\'a}n Angulo and Horacio Almanza and Bel{\'e}n Borrego and Mar{\'i}a Zamora-Ceballos and Cast{\'o}n, {Jos{\'e} R.} and Ignacio Mena and Esther Blanco and Juan B{\'a}rcena",

note = "Publisher Copyright: {\textcopyright} 2020 The Author(s).",

year = "2020",

month = may,

day = "1",

doi = "10.1186/s13567-020-00785-x",

language = "English",

volume = "51",

journal = "Veterinary Research",

issn = "0928-4249",

publisher = "BioMed Central Ltd.",

number = "1",

}

TY - JOUR

T1 - Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies

AU - Cubillos-Zapata, Carolina

AU - Angulo, Iván

AU - Almanza, Horacio

AU - Borrego, Belén

AU - Zamora-Ceballos, María

AU - Castón, José R.

AU - Mena, Ignacio

AU - Blanco, Esther

AU - Bárcena, Juan

PY - 2020/5/1

Y1 - 2020/5/1

N2 - We report the generation, characterization and epitope mapping of a panel of 26 monoclonal antibodies (MAbs) against the VP1 capsid protein of feline calicivirus (FCV). Two close but distinct linear epitopes were identified at the capsid outermost surface (P2 subdomain) of VP1, within the E5′HVR antigenic hypervariable region: one spanning amino acids 431-435 (PAGDY), highly conserved and recognized by non-neutralizing MAbs; and a second epitope spanning amino acids 445-451 (ITTANQY), highly variable and recognized by neutralizing MAbs. These antibodies might be valuable for diagnostic applications, as well as for further research in different aspects of the biology of FCV.

AB - We report the generation, characterization and epitope mapping of a panel of 26 monoclonal antibodies (MAbs) against the VP1 capsid protein of feline calicivirus (FCV). Two close but distinct linear epitopes were identified at the capsid outermost surface (P2 subdomain) of VP1, within the E5′HVR antigenic hypervariable region: one spanning amino acids 431-435 (PAGDY), highly conserved and recognized by non-neutralizing MAbs; and a second epitope spanning amino acids 445-451 (ITTANQY), highly variable and recognized by neutralizing MAbs. These antibodies might be valuable for diagnostic applications, as well as for further research in different aspects of the biology of FCV.

UR - http://www.scopus.com/inward/record.url?scp=85084382445&partnerID=8YFLogxK

U2 - 10.1186/s13567-020-00785-x

DO - 10.1186/s13567-020-00785-x

M3 - Article

C2 - 32357948

AN - SCOPUS:85084382445

SN - 0928-4249

VL - 51

JO - Veterinary Research

JF - Veterinary Research

IS - 1

M1 - 59

ER -

Precise location of linear epitopes on the capsid surface of feline calicivirus recognized by neutralizing and non-neutralizing monoclonal antibodies

Abstract

Access to Document

Fingerprint

Cite this