TY - JOUR
T1 - Pouchitis is associated with mucosal imbalance between interleukin-8 and interleukin-10
AU - Bulois, Philippe
AU - Tremaine, William J.
AU - Maunoury, Vincent
AU - Gambiez, Luc
AU - Hafraoui, Samira
AU - Leteurtre, Emmanuelle
AU - Cortot, Antoine
AU - Sandborn, William J.
AU - Colombel, Jean Frédéric
AU - Desreumaux, Pierre
PY - 2000
Y1 - 2000
N2 - Background: Mucosal lesions of pouchitis are characterized by a neutrophil infiltrate. Interleukin (IL)-8 is the main mediator involved in neutrophil recruitment and is down-regulated by IL-10. Aim: To look for an imbalance between IL-8 and IL-10 in patients with pouchitis. Patients/Methods: 18 patients having an ileoanal pouch for ulcerative colitis were studied. Eleven had pouchitis defined by the pouchitis disease activity index of ≥7 points and 7 had no history of pouchitis. Biopsies taken at the site of inflammation or in the normal mucosa were scored for the histologic lesions, the intensity of neutrophil infiltration, and the presence of crypt abscesses. Mucosal IL-8 and IL-10 mRNA were quantified by competitive polymerase chain reaction. Results: IL-8, IL-10, and IL-10/IL-8 mRNA were similar in patients with or without pouchitis. IL-8 mRNA levels were significantly higher in patients with a histologic score >2 (p = 0.01) and in patients with crypt abscesses (p = 0.01). IL-10/IL-8 mRNA was significantly lower in patients having a histologic score >2 (p = 0.019), a neutrophil infiltration ≥10%(p = 0.013), and crypt abscesses (p = 0.01). Conclusion: Histologic lesions of pouchitis are associated with a mucosal imbalance between IL-8 and IL-10. IL-10 could be proposed as a new treatment for pouchitis.
AB - Background: Mucosal lesions of pouchitis are characterized by a neutrophil infiltrate. Interleukin (IL)-8 is the main mediator involved in neutrophil recruitment and is down-regulated by IL-10. Aim: To look for an imbalance between IL-8 and IL-10 in patients with pouchitis. Patients/Methods: 18 patients having an ileoanal pouch for ulcerative colitis were studied. Eleven had pouchitis defined by the pouchitis disease activity index of ≥7 points and 7 had no history of pouchitis. Biopsies taken at the site of inflammation or in the normal mucosa were scored for the histologic lesions, the intensity of neutrophil infiltration, and the presence of crypt abscesses. Mucosal IL-8 and IL-10 mRNA were quantified by competitive polymerase chain reaction. Results: IL-8, IL-10, and IL-10/IL-8 mRNA were similar in patients with or without pouchitis. IL-8 mRNA levels were significantly higher in patients with a histologic score >2 (p = 0.01) and in patients with crypt abscesses (p = 0.01). IL-10/IL-8 mRNA was significantly lower in patients having a histologic score >2 (p = 0.019), a neutrophil infiltration ≥10%(p = 0.013), and crypt abscesses (p = 0.01). Conclusion: Histologic lesions of pouchitis are associated with a mucosal imbalance between IL-8 and IL-10. IL-10 could be proposed as a new treatment for pouchitis.
KW - Interleukin-10
KW - Interleukin-8
KW - Pouchitis
KW - Ulcerative colitis
UR - http://www.scopus.com/inward/record.url?scp=17144447589&partnerID=8YFLogxK
U2 - 10.1097/00054725-200008000-00001
DO - 10.1097/00054725-200008000-00001
M3 - Article
C2 - 10961587
AN - SCOPUS:17144447589
SN - 1078-0998
VL - 6
SP - 157
EP - 164
JO - Inflammatory Bowel Diseases
JF - Inflammatory Bowel Diseases
IS - 3
ER -