TY - JOUR
T1 - Potential role of increased matrix metalloproteinase-2 (MMP2) transcription in impaired adipogenesis in type 2 diabetes mellitus
AU - Dubois, Severine G.
AU - Tchoukalova, Yourka D.
AU - Heilbronn, Leonie K.
AU - Albu, Jeanine B.
AU - Kelley, David E.
AU - Smith, Steven R.
AU - Fang, Xiaobing
AU - Ravussin, Eric
N1 - Funding Information:
We thank the rest of members of the Look AHEAD adipose research groups for their contribution to this project. The study was funded by DK60412 (E.R.) but also supported by the University of Pittsburgh Obesity & Nutrition Research Center (P3-DK46204), the University of Pittsburgh General Clinical Research Center (MO1-RR000056), the Pennington Biomedical Research Center Clinical Nutrition Research Unit (P30 DK072476), and the Columbia University Diabetes and Endocrinology Research Center (P30 DK63608).
PY - 2008/3/21
Y1 - 2008/3/21
N2 - We measured gene expression of paracrine regulators involved in adipocyte differentiation within the stromovascular fraction of abdominal subcutaneous adipose tissue from obese individuals with (n = 30) and without (n = 18) type 2 diabetes mellitus (T2DM). Despite similar adiposity by design, subjects with T2DM had larger adipocytes (0.92 ± 0.28 vs. 0.75 ± 0.17 μl, p < 0.05) than controls. Gene expression of the adipogenic marker aP2 was lower (0.35 ± 0.16 vs. 0.58 ± 0.27 arbitrary units, p < 0.05) whereas the expression of matricellular peptidase, MMP2 was higher (1.65 ± 0.17 vs. 1.27 ± 0.21, p = 0.02) in T2DM vs. controls. The gene expression levels between the aP2 and MMP2 were inversely correlated (r = -0.32, p = 0.03). We conclude that early steps of adipogenesis may be impaired in T2DM independently of obesity due, in part, to an upregulation of the MMP2 transcription.
AB - We measured gene expression of paracrine regulators involved in adipocyte differentiation within the stromovascular fraction of abdominal subcutaneous adipose tissue from obese individuals with (n = 30) and without (n = 18) type 2 diabetes mellitus (T2DM). Despite similar adiposity by design, subjects with T2DM had larger adipocytes (0.92 ± 0.28 vs. 0.75 ± 0.17 μl, p < 0.05) than controls. Gene expression of the adipogenic marker aP2 was lower (0.35 ± 0.16 vs. 0.58 ± 0.27 arbitrary units, p < 0.05) whereas the expression of matricellular peptidase, MMP2 was higher (1.65 ± 0.17 vs. 1.27 ± 0.21, p = 0.02) in T2DM vs. controls. The gene expression levels between the aP2 and MMP2 were inversely correlated (r = -0.32, p = 0.03). We conclude that early steps of adipogenesis may be impaired in T2DM independently of obesity due, in part, to an upregulation of the MMP2 transcription.
KW - Adipocyte
KW - Fatty acid binding protein aP2
KW - Matrix metalloproteinase
KW - Obesity
KW - Type 2 diabetes
UR - http://www.scopus.com/inward/record.url?scp=38649122713&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2007.12.180
DO - 10.1016/j.bbrc.2007.12.180
M3 - Article
C2 - 18182154
AN - SCOPUS:38649122713
SN - 0006-291X
VL - 367
SP - 725
EP - 728
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -