Potent neutralization of primary human immunodeficiency virus clade C isolates with a synergistic combination of human monoclonal antibodies raised against clade B

W. Xu; B. A. Smith-Franklin; P. L. Li; C. Wood; J. He; Q. Du; G. J. Bhat; C. Kankasa; H. Katinger; L. A. Cavacini; M. R. Posner; D. R. Burton; T. C. Chou; R. M. Ruprecht

Potent neutralization of primary human immunodeficiency virus clade C isolates with a synergistic combination of human monoclonal antibodies raised against clade B

W. Xu, B. A. Smith-Franklin, P. L. Li, C. Wood, J. He, Q. Du, G. J. Bhat, C. Kankasa, H. Katinger, L. A. Cavacini, M. R. Posner, D. R. Burton, T. C. Chou, R. M. Ruprecht

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Objectives: We investigated the ability of several human neutralizing monoclonal antibodies (mAbs), originally raised against human immunodeficiency virus (HIV) clade B isolates, to neutralize primary clade C isolates as single agents and in combination. Study Design/Methods: HIV clade C isolates from five different countries were tested for susceptibility to neutralization by anti-clade B mAbs in human peripheral blood mononuclear cells. Monoclonal antibody combinations were evaluated for possible synergy. Results: All 20 primary HIV clade C isolates could be neutralized 97.5% to 100% by a quadruple combination of mAbs IgG1b12, 2G12, 2F5, and 4E10. These mAbs recognized conserved epitopes and were highly synergistic, resulting in strong cross-clade neutralization. Conclusions: In our previous experiment, a synergistic combination of human neutralizing mAbs protected all macaque neonates against oral challenge with a simian-human immunodeficiency virus encoding HIV env. Together, our data suggest that passive immunization with currently available anti-clade B mAbs could play a role in preventing HIV clade C transmission through breastfeeding.

Original language	English
Pages (from-to)	55-61
Number of pages	7
Journal	Journal of Human Virology
Volume	4
Issue number	2
State	Published - 2001
Externally published	Yes

Keywords

HIV-1 clade C
Human neutralizing monoclonal antibodies
Neutralization
Passive immunization

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Xu, W., Smith-Franklin, B. A., Li, P. L., Wood, C., He, J., Du, Q., Bhat, G. J., Kankasa, C., Katinger, H., Cavacini, L. A., Posner, M. R., Burton, D. R., Chou, T. C., & Ruprecht, R. M. (2001). Potent neutralization of primary human immunodeficiency virus clade C isolates with a synergistic combination of human monoclonal antibodies raised against clade B. Journal of Human Virology, 4(2), 55-61.

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title = "Potent neutralization of primary human immunodeficiency virus clade C isolates with a synergistic combination of human monoclonal antibodies raised against clade B",

abstract = "Objectives: We investigated the ability of several human neutralizing monoclonal antibodies (mAbs), originally raised against human immunodeficiency virus (HIV) clade B isolates, to neutralize primary clade C isolates as single agents and in combination. Study Design/Methods: HIV clade C isolates from five different countries were tested for susceptibility to neutralization by anti-clade B mAbs in human peripheral blood mononuclear cells. Monoclonal antibody combinations were evaluated for possible synergy. Results: All 20 primary HIV clade C isolates could be neutralized 97.5% to 100% by a quadruple combination of mAbs IgG1b12, 2G12, 2F5, and 4E10. These mAbs recognized conserved epitopes and were highly synergistic, resulting in strong cross-clade neutralization. Conclusions: In our previous experiment, a synergistic combination of human neutralizing mAbs protected all macaque neonates against oral challenge with a simian-human immunodeficiency virus encoding HIV env. Together, our data suggest that passive immunization with currently available anti-clade B mAbs could play a role in preventing HIV clade C transmission through breastfeeding.",

keywords = "HIV-1 clade C, Human neutralizing monoclonal antibodies, Neutralization, Passive immunization",

author = "W. Xu and Smith-Franklin, {B. A.} and Li, {P. L.} and C. Wood and J. He and Q. Du and Bhat, {G. J.} and C. Kankasa and H. Katinger and Cavacini, {L. A.} and Posner, {M. R.} and Burton, {D. R.} and Chou, {T. C.} and Ruprecht, {R. M.}",

year = "2001",

language = "English",

volume = "4",

pages = "55--61",

journal = "Journal of Human Virology",

issn = "1090-9508",

publisher = "Lippincott Williams and Wilkins Ltd.",

number = "2",

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Xu, W, Smith-Franklin, BA, Li, PL, Wood, C, He, J, Du, Q, Bhat, GJ, Kankasa, C, Katinger, H, Cavacini, LA, Posner, MR, Burton, DR, Chou, TC & Ruprecht, RM 2001, 'Potent neutralization of primary human immunodeficiency virus clade C isolates with a synergistic combination of human monoclonal antibodies raised against clade B', Journal of Human Virology, vol. 4, no. 2, pp. 55-61.

TY - JOUR

T1 - Potent neutralization of primary human immunodeficiency virus clade C isolates with a synergistic combination of human monoclonal antibodies raised against clade B

AU - Xu, W.

AU - Smith-Franklin, B. A.

AU - Li, P. L.

AU - Wood, C.

AU - He, J.

AU - Du, Q.

AU - Bhat, G. J.

AU - Kankasa, C.

AU - Katinger, H.

AU - Cavacini, L. A.

AU - Posner, M. R.

AU - Burton, D. R.

AU - Chou, T. C.

AU - Ruprecht, R. M.

PY - 2001

Y1 - 2001

N2 - Objectives: We investigated the ability of several human neutralizing monoclonal antibodies (mAbs), originally raised against human immunodeficiency virus (HIV) clade B isolates, to neutralize primary clade C isolates as single agents and in combination. Study Design/Methods: HIV clade C isolates from five different countries were tested for susceptibility to neutralization by anti-clade B mAbs in human peripheral blood mononuclear cells. Monoclonal antibody combinations were evaluated for possible synergy. Results: All 20 primary HIV clade C isolates could be neutralized 97.5% to 100% by a quadruple combination of mAbs IgG1b12, 2G12, 2F5, and 4E10. These mAbs recognized conserved epitopes and were highly synergistic, resulting in strong cross-clade neutralization. Conclusions: In our previous experiment, a synergistic combination of human neutralizing mAbs protected all macaque neonates against oral challenge with a simian-human immunodeficiency virus encoding HIV env. Together, our data suggest that passive immunization with currently available anti-clade B mAbs could play a role in preventing HIV clade C transmission through breastfeeding.

AB - Objectives: We investigated the ability of several human neutralizing monoclonal antibodies (mAbs), originally raised against human immunodeficiency virus (HIV) clade B isolates, to neutralize primary clade C isolates as single agents and in combination. Study Design/Methods: HIV clade C isolates from five different countries were tested for susceptibility to neutralization by anti-clade B mAbs in human peripheral blood mononuclear cells. Monoclonal antibody combinations were evaluated for possible synergy. Results: All 20 primary HIV clade C isolates could be neutralized 97.5% to 100% by a quadruple combination of mAbs IgG1b12, 2G12, 2F5, and 4E10. These mAbs recognized conserved epitopes and were highly synergistic, resulting in strong cross-clade neutralization. Conclusions: In our previous experiment, a synergistic combination of human neutralizing mAbs protected all macaque neonates against oral challenge with a simian-human immunodeficiency virus encoding HIV env. Together, our data suggest that passive immunization with currently available anti-clade B mAbs could play a role in preventing HIV clade C transmission through breastfeeding.

KW - HIV-1 clade C

KW - Human neutralizing monoclonal antibodies

KW - Neutralization

KW - Passive immunization

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M3 - Article

C2 - 11437315

AN - SCOPUS:0034971817

SN - 1090-9508

VL - 4

SP - 55

EP - 61

JO - Journal of Human Virology

JF - Journal of Human Virology

IS - 2

ER -

Potent neutralization of primary human immunodeficiency virus clade C isolates with a synergistic combination of human monoclonal antibodies raised against clade B

Abstract

Keywords

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