Pooling strategies for establishing physical genome maps using FISH

Fengzhu Sun, Gary Benson, Norman Arnheim, Michael S. Waterman

Research output: Contribution to conferencePaperpeer-review

Abstract

Often, in biological studies, it is necessary to identify an organism's chromosomes. In some organisms the individual chromosomes can be identified by staining procedures while many other species have a very large number of chromosomes, often of similar size, which defy identification by traditional staining methods. We have devised strategies, based on fluorescent in situ hybridization (FISH), which allow the assignment of a preset number of probes to each chromosome without prior chromosome identification. By hybridizing mixtures of probes labeled with different colored fluorescent molecules the chromosomal origin of each probe can be determined.

Original languageEnglish
Pages294-299
Number of pages6
DOIs
StatePublished - 1997
Externally publishedYes
EventProceedings of the 1997 1st Annual International Conference on Computational Molecular Biology, RECOMB - Santa Fe, NM, USA
Duration: 20 Jan 199723 Jan 1997

Conference

ConferenceProceedings of the 1997 1st Annual International Conference on Computational Molecular Biology, RECOMB
CitySanta Fe, NM, USA
Period20/01/9723/01/97

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