Pooled shRNA screenings: Experimental approach

Ruth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

12 Scopus citations


RNA interference (RNAi) has emerged as a powerful genetic strategy to functionally interrogate the entire genome by loss-of-function studies. In the last years, several arrayed shRNA libraries that can silence almost all the human genome have been developed. The generation of new and more efficient shRNA plasmids has allowed performing genetic screens in a pooled fashion and provides the opportunity to investigate the entire genomefinding relevant genes to any biological process. In this chapter, the pipeline and methods to perform a pooled shRNA screen are discussed.

Original languageEnglish
Title of host publicationPancreatic Cancer
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages18
ISBN (Print)9781627032865
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Genome-wide
  • Pooled screen
  • ShRNA


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