Polypeptide-based gold nanoshells for photothermal therapy

Kristine M. Mayle, Kathryn R. Dern, Vincent K. Wong, Shijun Sung, Ke Ding, April R. Rodriguez, Zachary Taylor, Z. Hong Zhou, Warren S. Grundfest, Timothy J. Deming, Daniel T. Kamei

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Targeted killing of cancer cells by engineered nanoparticles holds great promise for noninvasive photothermal therapy applications. We present the design and generation of a novel class of gold nanoshells with cores composed of selfassembled block copolypeptide vesicles with photothermal properties. Specifically, poly(L-lysine)60-block-poly(L-leucine)20 (K60L20) block copolypeptide vesicles coated with a thin layer of gold demonstrate enhanced absorption of light due to surface plasmon resonance (SPR) in the near-infrared range. We show that the polypeptide-based K60L20 gold nanoshells have low toxicity in the absence of laser exposure, significant heat generation upon exposure to near-infrared light, and, as a result, localized cytotoxicity within the region of laser irradiation in vitro. To gain a better understanding of our gold nanoshells in the context of photothermal therapy, we developed a comprehensive mathematical model for heat transfer and experimentally validated this model by predicting the temperature as a function of time and position in our experimental setup. This model can be used to predict which parameters of our gold nanoshells can be manipulated to improve heat generation for tumor destruction. To our knowledge, our results represent the first ever use of block copolypeptide vesicles as the core material of gold nanoshells.

Original languageEnglish
Pages (from-to)18-25
Number of pages8
JournalSLAS Technology
Volume22
Issue number1
DOIs
StatePublished - Feb 2017
Externally publishedYes

Keywords

  • Cancer
  • Gold nanoshells
  • Nanoparticles
  • Photothermal therapies
  • Polypeptide vesicles

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