Polymorphic microsatellite markers in the olive fly, Bactrocera oleae

Bactrocera oleae is the single most important insect pest of the olive fruit, causing extensive of the losses in the olive production annually. Nonetheless, there has never been an analysis of the homogeneity of B. oleae populations, the extent of gene flow, the genetic differentiation and/or reproductive isolation of marginal populations or the nature of the invasive populations in newly established olive tree cultivations. Here we describe the development of 10 novel polymorphic microsatellite markers that can be used in the analysis of natural B. oleae populations as well as to create a basis for its genome analysis.