Polychromatic angiography for the assessment of VEGF-induced BRB dysfunction in the rabbit retina

Samir R. Tari, Maher Youssif, C. Michael Samson, Robert L. Harris, Cheng Mao Lin, Uday B. Kompella, David A. Antonetti, Gaetano R. Barile

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Purpose. To determine the utility of polychromatic angiography (PCA) in the assessment of VEGF-induced blood retinal barrier (BRB) dysfunction in rabbits. Methods. Twenty-six eyes of 24 Dutch Belted rabbits were injected intravitreally with 1.25 lg (group A, n = 5), 10 lg (group C, n = 7), or 4 lg (group B, n = 6; group D, n = 4; and group E, n = 4) of VEGF on day 0. Groups D and E were also injected intravitreally with 1.25 lg and 12.5 lg bevacizumab, respectively, on day 2. On days 0, 2, 4, 7, 11, and 14, PCA was performed using a contrast agent mixture composed of fluorescein sodium, indocyanine green, PCM102, and PCM107 and imaged with a modified fundus camera. PCA scores were based on detected leaking fluorophores. Results. On day 7, there was a statistically significant difference between PCA scores of group A (0.6±0.89) and both groups B (2.67±1.37, P = 0.0154) and C (3.33±0.52, P = 0.00085). There was also a statistically significant difference between groups B and E (PCA score 0.75±0.96, P = 0.032) on day 7. On day 11, there was statistically significant difference between group C (1.80±1.1) and both groups A (0, P = 0.021) and B (0.33±0.52, P = 0.037). Conclusions. A differential response to both increasing VEGF dose and administration of bevacizumab could be discerned using the PCA. PCA allowed stratification of VEGF-induced BRB dysfunction and inhibitory effects of bevacizumab therapy in the rabbit retina.

Original languageEnglish
Pages (from-to)5550-5558
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume54
Issue number8
DOIs
StatePublished - 2013
Externally publishedYes

Keywords

  • Blood retinal barrier
  • Polychromatic angiography
  • Retina
  • Retinal imaging

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