Platelet-derived growth factor B chain homodimer enhances chemotaxis and DNA synthesis in normal osteoblast-like cells (MC3T3-E1)

Tatsuo Tsukamoto; Toshimitu Matsui; Massaki Fukase; Takuo Fujita

doi:10.1016/0006-291X(91)91629-Q

Platelet-derived growth factor B chain homodimer enhances chemotaxis and DNA synthesis in normal osteoblast-like cells (MC3T3-E1)

Tatsuo Tsukamoto, Toshimitu Matsui, Massaki Fukase, Takuo Fujita

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Abstract

We demonstrate that recombinant platelet-derived growth factor B chain homodimer (PDGF-BB) could induce both the chemotactic activity and the DNA synthesis in a normal osteoblast-like cell line (MC3T3-E1). Cell migration toward recombinant human PDGF-BB was observed with the modified Boyden chamber technique. Maximum chemotaxis was exhibited at 25 ng/ml of PDGF-BB. DNA synthesis as indicated by [³H]-thymidine incorporation was also enhanced about 4-fold at 25-100 ng/ml PDGF-BB. Our results suggested that PDGF might be one of the candidates among local coupling factors for bone remodeling.

Original language	English
Pages (from-to)	745-751
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	175
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(91)91629-Q
State	Published - 29 Mar 1991
Externally published	Yes

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abstract = "We demonstrate that recombinant platelet-derived growth factor B chain homodimer (PDGF-BB) could induce both the chemotactic activity and the DNA synthesis in a normal osteoblast-like cell line (MC3T3-E1). Cell migration toward recombinant human PDGF-BB was observed with the modified Boyden chamber technique. Maximum chemotaxis was exhibited at 25 ng/ml of PDGF-BB. DNA synthesis as indicated by [3H]-thymidine incorporation was also enhanced about 4-fold at 25-100 ng/ml PDGF-BB. Our results suggested that PDGF might be one of the candidates among local coupling factors for bone remodeling.",

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AU - Tsukamoto, Tatsuo

AU - Matsui, Toshimitu

AU - Fukase, Massaki

AU - Fujita, Takuo

PY - 1991/3/29

Y1 - 1991/3/29

N2 - We demonstrate that recombinant platelet-derived growth factor B chain homodimer (PDGF-BB) could induce both the chemotactic activity and the DNA synthesis in a normal osteoblast-like cell line (MC3T3-E1). Cell migration toward recombinant human PDGF-BB was observed with the modified Boyden chamber technique. Maximum chemotaxis was exhibited at 25 ng/ml of PDGF-BB. DNA synthesis as indicated by [3H]-thymidine incorporation was also enhanced about 4-fold at 25-100 ng/ml PDGF-BB. Our results suggested that PDGF might be one of the candidates among local coupling factors for bone remodeling.

AB - We demonstrate that recombinant platelet-derived growth factor B chain homodimer (PDGF-BB) could induce both the chemotactic activity and the DNA synthesis in a normal osteoblast-like cell line (MC3T3-E1). Cell migration toward recombinant human PDGF-BB was observed with the modified Boyden chamber technique. Maximum chemotaxis was exhibited at 25 ng/ml of PDGF-BB. DNA synthesis as indicated by [3H]-thymidine incorporation was also enhanced about 4-fold at 25-100 ng/ml PDGF-BB. Our results suggested that PDGF might be one of the candidates among local coupling factors for bone remodeling.

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Platelet-derived growth factor B chain homodimer enhances chemotaxis and DNA synthesis in normal osteoblast-like cells (MC3T3-E1)

Abstract

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