TY - JOUR
T1 - Plant lipid transfer protein allergens
T2 - No cross-reactivity between those from foods and olive and Parietaria pollen
AU - Tordesillas, Leticia
AU - Sirvent, Sofía
AU - Díaz-Perales, Araceli
AU - Villalba, Mayte
AU - Cuesta-Herranz, Javier
AU - Rodríguez, Rosalía
AU - Salcedo, Gabriel
PY - 2011/10
Y1 - 2011/10
N2 - Background: Cross-reactivity among plant food allergens belonging to the nonspecific lipid transfer protein (LTP) family is well known. In contrast, the relationship among these allergens and their putative homologs from olive (Ole e 7) and Parietaria (Par j 1) pollen has not been clarified. Methods: Sera with specific IgE to LTP allergens were obtained from peach-, mustard- and olive pollen-allergic patients. Purified LTP allergens from foods (peach, apple, mustard and wheat) and pollens (olive, mugwort and Parietaria) were tested by ELISA and ELISA-inhibition assays. Results: Plant food LTP-allergic patients showed a significantly higher number of sera (89-100 vs. 33-64%) with specific IgE and mean specific IgE levels (0.30-1.56 vs. 0.21-0.34 OD units) to the 4 food LTP allergens tested than to olive Ole e 7 and Parietaria Par j 1 pollen. ELISA-inhibition assays indicated cross-inhibition between food LTP allergens but no cross-reactivity between these allergens and Ole e 7 and Par j 1, or, even more, between the LTP allergens from olive and Parietaria pollen. Conclusions: LTP allergens from olive and Parietaria pollen cross-react neither with allergenic LTPs from plant foods nor between themselves. Therefore, both pollens do not seem to be related with the LTP syndrome.
AB - Background: Cross-reactivity among plant food allergens belonging to the nonspecific lipid transfer protein (LTP) family is well known. In contrast, the relationship among these allergens and their putative homologs from olive (Ole e 7) and Parietaria (Par j 1) pollen has not been clarified. Methods: Sera with specific IgE to LTP allergens were obtained from peach-, mustard- and olive pollen-allergic patients. Purified LTP allergens from foods (peach, apple, mustard and wheat) and pollens (olive, mugwort and Parietaria) were tested by ELISA and ELISA-inhibition assays. Results: Plant food LTP-allergic patients showed a significantly higher number of sera (89-100 vs. 33-64%) with specific IgE and mean specific IgE levels (0.30-1.56 vs. 0.21-0.34 OD units) to the 4 food LTP allergens tested than to olive Ole e 7 and Parietaria Par j 1 pollen. ELISA-inhibition assays indicated cross-inhibition between food LTP allergens but no cross-reactivity between these allergens and Ole e 7 and Par j 1, or, even more, between the LTP allergens from olive and Parietaria pollen. Conclusions: LTP allergens from olive and Parietaria pollen cross-react neither with allergenic LTPs from plant foods nor between themselves. Therefore, both pollens do not seem to be related with the LTP syndrome.
KW - Cross-reactivity
KW - Lipid transfer protein
KW - Olive
KW - Panallergen
KW - Parietaria
KW - Peach
KW - Pollen-food cross-reaction
UR - http://www.scopus.com/inward/record.url?scp=79959681934&partnerID=8YFLogxK
U2 - 10.1159/000323503
DO - 10.1159/000323503
M3 - Article
C2 - 21720174
AN - SCOPUS:79959681934
SN - 1018-2438
VL - 156
SP - 291
EP - 296
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
IS - 3
ER -