TY - JOUR
T1 - Placental/umbilical cord blood for unrelated-donor bone marrow reconstitution
T2 - Relevance of nucleated red blood cells
AU - Stevens, Cladd E.
AU - Gladstone, Jessica
AU - Taylor, Patricia E.
AU - Scaradavou, Andromachi
AU - Migliaccio, Anna Rita
AU - Visser, Jan
AU - Ludy Dobrila, N.
AU - Carrier, Carmelita
AU - Cabbad, Michael
AU - Wernet, Peter
AU - Kurtzberg, Joanne
AU - Rubinstein, Pablo
PY - 2002/10/1
Y1 - 2002/10/1
N2 - Placental/umbilical cord blood (PCB) is a source of hematopoietic stem cells for bone marrow reconstitution. Engraftment speed and survival are related to the total nucleated cell (TNC) dose of the graft. This study explored the possible influence on engraftment of nucleated red blood cells (NRBCs) in the graft. Automated hematology analyzers were used to enumerate TNCs. NRBCs were counted by visual examination or by using an automated analyzer. Hematopoietic progenitor cells were enumerated as either colony-forming cells or CD34+ cells. Transplant centers reported on transplant outcome in 1112 patients given PCB grafts through September 2001. NRBCs correlated with progenitor cell numbers. Both white blood cell and NRBC dose were independently predictive of myeloid engraftment speed. Because NRBC dose predicted engraftment speed, inclusion of NRBCs in the TNC count does not reduce the effectiveness of the prefreezing TNC count as an index of the quality of a PCB unit as a graft. The correlation between the number of NRBCs and the number of hematopoietic progenitor cells probably reflects the involvement of early stem cells in erythroid responses.
AB - Placental/umbilical cord blood (PCB) is a source of hematopoietic stem cells for bone marrow reconstitution. Engraftment speed and survival are related to the total nucleated cell (TNC) dose of the graft. This study explored the possible influence on engraftment of nucleated red blood cells (NRBCs) in the graft. Automated hematology analyzers were used to enumerate TNCs. NRBCs were counted by visual examination or by using an automated analyzer. Hematopoietic progenitor cells were enumerated as either colony-forming cells or CD34+ cells. Transplant centers reported on transplant outcome in 1112 patients given PCB grafts through September 2001. NRBCs correlated with progenitor cell numbers. Both white blood cell and NRBC dose were independently predictive of myeloid engraftment speed. Because NRBC dose predicted engraftment speed, inclusion of NRBCs in the TNC count does not reduce the effectiveness of the prefreezing TNC count as an index of the quality of a PCB unit as a graft. The correlation between the number of NRBCs and the number of hematopoietic progenitor cells probably reflects the involvement of early stem cells in erythroid responses.
UR - http://www.scopus.com/inward/record.url?scp=0036786949&partnerID=8YFLogxK
U2 - 10.1182/blood.V100.7.2662
DO - 10.1182/blood.V100.7.2662
M3 - Article
C2 - 12239183
AN - SCOPUS:0036786949
SN - 0006-4971
VL - 100
SP - 2662
EP - 2664
JO - Blood
JF - Blood
IS - 7
ER -