PiRNA production requires heterochromatin formation in drosophila

Prashanth Rangan, Colin D. Malone, Caryn Navarro, Sam P. Newbold, Patrick S. Hayes, Ravi Sachidanandam, Gregory J. Hannon, Ruth Lehmann

Research output: Contribution to journalArticlepeer-review

156 Scopus citations

Abstract

Protecting the genome from transposable element (TE) mobilization is critical for germline development. In Drosophila, Piwi proteins and their bound small RNAs (piRNAs) provide a potent defense against TE activity. TE targeting piRNAs are processed from TE-dense heterochromatic loci termed piRNA clusters. Although piRNA biogenesis from cluster precursors is beginning to be understood, little is known about piRNA cluster transcriptional regulation. Here, we show that deposition of histone 3 lysine 9 by the methyltransferase dSETDB1 (egg) is required for piRNA cluster transcription. In the absence of dSETDB1, cluster precursor transcription collapses in germline and somatic gonadal cells and TEs are activated, resulting in germline loss and a block in germline stem cell differentiation. We propose that heterochromatin protects the germline by activating the piRNA pathway.

Original languageEnglish
Pages (from-to)1373-1379
Number of pages7
JournalCurrent Biology
Volume21
Issue number16
DOIs
StatePublished - 23 Aug 2011

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