Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel

Douglas I. Walker; Kurt D. Pennell; Karan Uppal; Xiaoyan Xia; Philip K. Hopke; Mark J. Utell; Richard P. Phipps; Patricia J. Sime; Patricia Rohrbeck; Timothy M. Mallon; Dean P. Jones

doi:10.1097/JOM.0000000000000772

Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel

Douglas I. Walker, Kurt D. Pennell, Karan Uppal, Xiaoyan Xia, Philip K. Hopke, Mark J. Utell, Richard P. Phipps, Patricia J. Sime, Patricia Rohrbeck, Timothy M. Mallon, Dean P. Jones

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Objective: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. Methods: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. Results: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. Conclusion: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.

Original language	English
Pages (from-to)	S44-S52
Journal	Journal of Occupational and Environmental Medicine
Volume	58
Issue number	8S
DOIs	https://doi.org/10.1097/JOM.0000000000000772
State	Published - 1 Aug 2016
Externally published	Yes

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title = "Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel",

abstract = "Objective: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. Methods: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. Results: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. Conclusion: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.",

author = "Walker, {Douglas I.} and Pennell, {Kurt D.} and Karan Uppal and Xiaoyan Xia and Hopke, {Philip K.} and Utell, {Mark J.} and Phipps, {Richard P.} and Sime, {Patricia J.} and Patricia Rohrbeck and Mallon, {Timothy M.} and Jones, {Dean P.}",

note = "Publisher Copyright: {\textcopyright} 2016 American College of Occupational and Environmental Medicine.",

year = "2016",

month = aug,

day = "1",

doi = "10.1097/JOM.0000000000000772",

language = "English",

volume = "58",

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journal = "Journal of Occupational and Environmental Medicine",

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TY - JOUR

T1 - Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel

AU - Walker, Douglas I.

AU - Pennell, Kurt D.

AU - Uppal, Karan

AU - Xia, Xiaoyan

AU - Hopke, Philip K.

AU - Utell, Mark J.

AU - Phipps, Richard P.

AU - Sime, Patricia J.

AU - Rohrbeck, Patricia

AU - Mallon, Timothy M.

AU - Jones, Dean P.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Objective: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. Methods: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. Results: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. Conclusion: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.

AB - Objective: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. Methods: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. Results: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. Conclusion: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.

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VL - 58

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JO - Journal of Occupational and Environmental Medicine

JF - Journal of Occupational and Environmental Medicine

IS - 8S

ER -

Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel

Abstract

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