Physical mapping of the human connexin 40 (GJA5), flavin-containing monooxygenase 5, and natriuretic peptide receptor A genes on 1q21

Bruce D. Gelb, Jian Zhang, Philip D. Cotter, Irina F. Gershin, Robert J. Desnick

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The human connexin 40 gene (Cx40; HGMW-approved symbol GJAS), a gap junction protein, was mapped previously to 1pter-q12 by analysis of somatic cell hybrids. In the development of a yeast artificial chromosome (YAC) contig at 1q21, a YAC end clone contained the entire 5'-untranslated region and 42 bases of coding region of the Cx40 gene. Using a sequence-tagged site (STS) developed from this sequence as well as amplimers for the natriuretic peptide receptor A (NPR1) and flavin-containing monooxygenase 5 (FMO5) genes, the order NPR1-FMO5-Cx40 was established, with Cx40 being the most telometic. Single-color FISH using PAC clones containing the Cx40 and NPR1 STSs localized these genes to 1q21.1.

Original languageEnglish
Pages (from-to)409-411
Number of pages3
JournalGenomics
Volume39
Issue number3
DOIs
StatePublished - 1 Feb 1997

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