Physical mapping and cloning of RAD56

David P. Mathiasen; Irene Gallina; Susanne M. Germann; Wissam Hamou; Morgane Eléouët; Sara Thodberg; Nadine Eckert-Boulet; John Game; Michael Lisby

doi:10.1016/j.gene.2013.01.044

Physical mapping and cloning of RAD56

David P. Mathiasen
, Irene Gallina
, Susanne M. Germann
, Wissam Hamou
, Morgane Eléouët
, Sara Thodberg
, Nadine Eckert-Boulet
, John Game
, Michael Lisby

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Here we report the physical mapping of the rad56-1 mutation to the NAT3 gene, which encodes the catalytic subunit of the NatB N-terminal acetyltransferase in Saccharomyces cerevisiae. Mutation of RAD56 causes sensitivity to X-rays, methyl methanesulfonate, zeocin, camptothecin and hydroxyurea, but not to UV light, suggesting that N-terminal acetylation of specific DNA repair proteins is important for efficient DNA repair.

Original language	English
Pages (from-to)	182-186
Number of pages	5
Journal	Gene
Volume	519
Issue number	1
DOIs	https://doi.org/10.1016/j.gene.2013.01.044
State	Published - 25 Apr 2013

Keywords

Acetylation
DNA repair
Saccharomyces cerevisiae

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10.1016/j.gene.2013.01.044

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title = "Physical mapping and cloning of RAD56",

abstract = "Here we report the physical mapping of the rad56-1 mutation to the NAT3 gene, which encodes the catalytic subunit of the NatB N-terminal acetyltransferase in Saccharomyces cerevisiae. Mutation of RAD56 causes sensitivity to X-rays, methyl methanesulfonate, zeocin, camptothecin and hydroxyurea, but not to UV light, suggesting that N-terminal acetylation of specific DNA repair proteins is important for efficient DNA repair.",

keywords = "Acetylation, DNA repair, Saccharomyces cerevisiae",

author = "Mathiasen, \{David P.\} and Irene Gallina and Germann, \{Susanne M.\} and Wissam Hamou and Morgane El{\'e}ou{\"e}t and Sara Thodberg and Nadine Eckert-Boulet and John Game and Michael Lisby",

note = "Funding Information: We thank members of the Lisby and Holmberg laboratories for helpful discussions on this work, and Rodney Rothstein, Hannah Klein and Ian Hickson for sharing reagents. This work was supported by The Danish Agency for Science, Technology and Innovation , the Villum Kann Rasmussen Foundation , the Lundbeck Foundation , the European Research Council (ERC) to M.L., I.G. and D.P.M., and the Deutscher Akademischer Auslandsdienst (DAAD) scholarship to S.M.G. We are grateful to the NBRP for providing the rad56-1 strain.",

year = "2013",

month = apr,

day = "25",

doi = "10.1016/j.gene.2013.01.044",

language = "English",

volume = "519",

pages = "182--186",

journal = "Gene",

issn = "0378-1119",

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TY - JOUR

T1 - Physical mapping and cloning of RAD56

AU - Mathiasen, David P.

AU - Gallina, Irene

AU - Germann, Susanne M.

AU - Hamou, Wissam

AU - Eléouët, Morgane

AU - Thodberg, Sara

AU - Eckert-Boulet, Nadine

AU - Game, John

AU - Lisby, Michael

N1 - Funding Information: We thank members of the Lisby and Holmberg laboratories for helpful discussions on this work, and Rodney Rothstein, Hannah Klein and Ian Hickson for sharing reagents. This work was supported by The Danish Agency for Science, Technology and Innovation , the Villum Kann Rasmussen Foundation , the Lundbeck Foundation , the European Research Council (ERC) to M.L., I.G. and D.P.M., and the Deutscher Akademischer Auslandsdienst (DAAD) scholarship to S.M.G. We are grateful to the NBRP for providing the rad56-1 strain.

PY - 2013/4/25

Y1 - 2013/4/25

N2 - Here we report the physical mapping of the rad56-1 mutation to the NAT3 gene, which encodes the catalytic subunit of the NatB N-terminal acetyltransferase in Saccharomyces cerevisiae. Mutation of RAD56 causes sensitivity to X-rays, methyl methanesulfonate, zeocin, camptothecin and hydroxyurea, but not to UV light, suggesting that N-terminal acetylation of specific DNA repair proteins is important for efficient DNA repair.

AB - Here we report the physical mapping of the rad56-1 mutation to the NAT3 gene, which encodes the catalytic subunit of the NatB N-terminal acetyltransferase in Saccharomyces cerevisiae. Mutation of RAD56 causes sensitivity to X-rays, methyl methanesulfonate, zeocin, camptothecin and hydroxyurea, but not to UV light, suggesting that N-terminal acetylation of specific DNA repair proteins is important for efficient DNA repair.

KW - Acetylation

KW - DNA repair

KW - Saccharomyces cerevisiae

UR - https://www.scopus.com/pages/publications/84875382579

U2 - 10.1016/j.gene.2013.01.044

DO - 10.1016/j.gene.2013.01.044

M3 - Article

C2 - 23403232

AN - SCOPUS:84875382579

SN - 0378-1119

VL - 519

SP - 182

EP - 186

JO - Gene

JF - Gene

IS - 1

ER -

Physical mapping and cloning of RAD56

Abstract

Keywords

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