Physical mapping and cloning of RAD56

David P. Mathiasen, Irene Gallina, Susanne M. Germann, Wissam Hamou, Morgane Eléouët, Sara Thodberg, Nadine Eckert-Boulet, John Game, Michael Lisby

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Here we report the physical mapping of the rad56-1 mutation to the NAT3 gene, which encodes the catalytic subunit of the NatB N-terminal acetyltransferase in Saccharomyces cerevisiae. Mutation of RAD56 causes sensitivity to X-rays, methyl methanesulfonate, zeocin, camptothecin and hydroxyurea, but not to UV light, suggesting that N-terminal acetylation of specific DNA repair proteins is important for efficient DNA repair.

Original languageEnglish
Pages (from-to)182-186
Number of pages5
JournalGene
Volume519
Issue number1
DOIs
StatePublished - 25 Apr 2013

Keywords

  • Acetylation
  • DNA repair
  • Saccharomyces cerevisiae

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