Phosphorylation of secreted forms of human β2-interferon/hepatocyte stimulating factor/interleukin-6

Lester T. May, Uma Santhanam, Stephen B. Tatter, Nina Bhardwaj, John Ghrayeb, Pravinkumar B. Sehgal

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


2-Interferon/hepatocyte stimulating factor/interleukin-6" (IFN-β2) has emerged as a major mediator of the plasma protein response to tissue injury (the acute phase response) in addition to its numerous effects on cells of the immune system. Human fibroblasts and monocytes induced with tumor necrosis factor, interleukin-1, bacterial lipopolysaccharide (endotoxin) or virus infection secrete multiple forms of differentially glycosylated IFN-β2 polypeptides: at least a doublet of molecular mass approximately 25 kD and a triplet of mass approximately 30 kD. We report that immunoprecipitation analyses of medium from [32P]orthophosphate- labeled cultures of induced fibroblasts carried out using a rabbit polyclonal antibody to recombinant E. coli-derived human IFN-β2 reveal that the secreted gp23-25 and gp28-30 forms of IFN-β2 are phosphorylated. IFN-β2 gp23-25 secreted by induced monocytes is phosphorylated whereas the monocytic gp28-30 is poorly labeled with [32P]orthophosphate suggesting tissue-specific differences in IFN-β2 phosphorylation. Phosphoamino acid analyses indicate that all of the detected phosphate is in phosphoserine residues. Furthermore, IFN-β2 can be completely dephosphorylated by alkaline phosphatase (E.C. No.; thus all of the phosphate label is in readily accesible sites. These observations suggest the possibility that differential phosphorylation of IFN-β2 forms may be a mechanism to modulate its functions in a tissue-specific manner.

Original languageEnglish
Pages (from-to)1144-1150
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - 16 May 1988
Externally publishedYes


Dive into the research topics of 'Phosphorylation of secreted forms of human β2-interferon/hepatocyte stimulating factor/interleukin-6'. Together they form a unique fingerprint.

Cite this