Phosphoenolpyruvate:Fructose phosphotransferase activity in whole cells and membrane vesicles of Arthrobacter pyridinolis

Ellen B. Wolfson; Mark E. Sobel; Terry A. Krulwich

doi:10.1016/0005-2744(73)90072-7

Phosphoenolpyruvate:Fructose phosphotransferase activity in whole cells and membrane vesicles of Arthrobacter pyridinolis

Ellen B. Wolfson, Mark E. Sobel, Terry A. Krulwich

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Mutants of Arthrobacter pyridiolis which were deficient in phosphoenol-pyruvate:fructose phosphotransferase activity were studied using in vitro complementation assays. In this way the strains were divided into those deficient in the inducible membrane-bound component and into thre groups which were deficient in different soluble components. One of the soluble components was inducible. Phosphoenolpyruvate:fructose phosphotransferase activity was also demonstrated in membrane vesicles prepared from fructose-grown A. pyridinolis. The activity was correlated with transport of fructose into the vesicles. Some kinetics parameters and temperature optima for the transport process in vesicles were studied.

Original language	English
Pages (from-to)	181-188
Number of pages	8
Journal	BBA - Enzymology
Volume	321
Issue number	1
DOIs	https://doi.org/10.1016/0005-2744(73)90072-7
State	Published - 15 Sep 1973
Externally published	Yes

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title = "Phosphoenolpyruvate:Fructose phosphotransferase activity in whole cells and membrane vesicles of Arthrobacter pyridinolis",

abstract = "Mutants of Arthrobacter pyridiolis which were deficient in phosphoenol-pyruvate:fructose phosphotransferase activity were studied using in vitro complementation assays. In this way the strains were divided into those deficient in the inducible membrane-bound component and into thre groups which were deficient in different soluble components. One of the soluble components was inducible. Phosphoenolpyruvate:fructose phosphotransferase activity was also demonstrated in membrane vesicles prepared from fructose-grown A. pyridinolis. The activity was correlated with transport of fructose into the vesicles. Some kinetics parameters and temperature optima for the transport process in vesicles were studied.",

author = "Wolfson, {Ellen B.} and Sobel, {Mark E.} and Krulwich, {Terry A.}",

note = "Funding Information: This work was supported by research grants GB-2o48I from the National Science Foufidation and AM-I4663o2 from the United States Public Health Service. We are grateful to Dr H. R. Kaback for his advice while we were initiating work with vesicles.",

year = "1973",

month = sep,

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doi = "10.1016/0005-2744(73)90072-7",

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T1 - Phosphoenolpyruvate:Fructose phosphotransferase activity in whole cells and membrane vesicles of Arthrobacter pyridinolis

AU - Wolfson, Ellen B.

AU - Sobel, Mark E.

AU - Krulwich, Terry A.

N1 - Funding Information: This work was supported by research grants GB-2o48I from the National Science Foufidation and AM-I4663o2 from the United States Public Health Service. We are grateful to Dr H. R. Kaback for his advice while we were initiating work with vesicles.

PY - 1973/9/15

Y1 - 1973/9/15

N2 - Mutants of Arthrobacter pyridiolis which were deficient in phosphoenol-pyruvate:fructose phosphotransferase activity were studied using in vitro complementation assays. In this way the strains were divided into those deficient in the inducible membrane-bound component and into thre groups which were deficient in different soluble components. One of the soluble components was inducible. Phosphoenolpyruvate:fructose phosphotransferase activity was also demonstrated in membrane vesicles prepared from fructose-grown A. pyridinolis. The activity was correlated with transport of fructose into the vesicles. Some kinetics parameters and temperature optima for the transport process in vesicles were studied.

AB - Mutants of Arthrobacter pyridiolis which were deficient in phosphoenol-pyruvate:fructose phosphotransferase activity were studied using in vitro complementation assays. In this way the strains were divided into those deficient in the inducible membrane-bound component and into thre groups which were deficient in different soluble components. One of the soluble components was inducible. Phosphoenolpyruvate:fructose phosphotransferase activity was also demonstrated in membrane vesicles prepared from fructose-grown A. pyridinolis. The activity was correlated with transport of fructose into the vesicles. Some kinetics parameters and temperature optima for the transport process in vesicles were studied.

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Phosphoenolpyruvate:Fructose phosphotransferase activity in whole cells and membrane vesicles of Arthrobacter pyridinolis

Abstract

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