TY - JOUR
T1 - Phorbol 12,13-dibutyrate (P(Bu)2)-treated human blood mononuclear cells bind to each other
AU - Patarroyo, Manuel
AU - Biberfeld, Peter
AU - Klein, Eva
AU - Klein, George
N1 - Funding Information:
This project has been supported by Grant 1 ROl CA 25250-03 awarded by the National Cancer Institute and by the Swedish Cancer Society. We thank Professors Bernard Weinstein and Abraham Loyter for helpful suggestions concerning the [3H]P(Bu)2 binding studies and use of various inhibitors. M. Patarroyo is the recipient of a fellowship from the Swedish Institute and the National University of Colombia. The technical assistance of Ms. Marianne Ekman is very much appreciated.
PY - 1983/1
Y1 - 1983/1
N2 - Treatment of human blood mononuclear cells with nanomolar concentrations of phorbol 12,13-dibutyrate (P(Bu)2) induced their aggregation. The phenomenon was seen within a few minutes and reached its maximum manifestation after 20 min. At this time, 20-30% of unfractionated and nylon wool-passed mononuclear leukocytes were in the aggregates. The influence of pretreatment with 2-deoxyglucose, NaN3, EDTA, cyclohexamide, or incubation at 4 °C on the phenomenon indicated that it is energy and temperature dependent, requires the presence of extracellular divalent cations, and is independent of protein synthesis. Nonaggregating 2-deoxyglucose- and NaN3-pretreated cells could still bind [3H]P(Bu)2 which rules out the possibility that the phorbol ester molecule acts as a bridge between the aggregated cells. Seventeen percent of the P(Bu)2-treated T-cell population bound untreated autologous and allogeneic cells. The binding property has a certain species specificity because only 4% of the cells interacted with mouse lymphocytes. At the ultrastructural level, the intercellular binding showed broad areas of surface contact (both between lymphocytes and lymphocyte-monocyte) and "trapping" by surface processes was not seen. Aggregated cells did not show cytopathogenic changes.
AB - Treatment of human blood mononuclear cells with nanomolar concentrations of phorbol 12,13-dibutyrate (P(Bu)2) induced their aggregation. The phenomenon was seen within a few minutes and reached its maximum manifestation after 20 min. At this time, 20-30% of unfractionated and nylon wool-passed mononuclear leukocytes were in the aggregates. The influence of pretreatment with 2-deoxyglucose, NaN3, EDTA, cyclohexamide, or incubation at 4 °C on the phenomenon indicated that it is energy and temperature dependent, requires the presence of extracellular divalent cations, and is independent of protein synthesis. Nonaggregating 2-deoxyglucose- and NaN3-pretreated cells could still bind [3H]P(Bu)2 which rules out the possibility that the phorbol ester molecule acts as a bridge between the aggregated cells. Seventeen percent of the P(Bu)2-treated T-cell population bound untreated autologous and allogeneic cells. The binding property has a certain species specificity because only 4% of the cells interacted with mouse lymphocytes. At the ultrastructural level, the intercellular binding showed broad areas of surface contact (both between lymphocytes and lymphocyte-monocyte) and "trapping" by surface processes was not seen. Aggregated cells did not show cytopathogenic changes.
UR - https://www.scopus.com/pages/publications/0020700622
U2 - 10.1016/0008-8749(83)90313-1
DO - 10.1016/0008-8749(83)90313-1
M3 - Article
C2 - 6402310
AN - SCOPUS:0020700622
SN - 0008-8749
VL - 75
SP - 144
EP - 153
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -