Phase I trial of intravenous and intraperitoneal administration of granulocyte-macrophage colony-stimulting factor

Guy C. Toner, Janice L. Gabrilove, Michael Gordon, John Crown, Ann A. Jakubowski, Barry Meisenberg, Carol Sheridan, Tom Boone, Martha E. Vincent, Maurie Markman

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Summary: To assess the toxicity, pharmacokinetics, and local and systemic effects of the intraperitoneal (i.p.) administraion of granulocyte-macrophage colony-stimulating factor (GM-CSF) at various dosages, 13 patinets were studied. GM-CSF was administered intravenously (i.v) for 5 consecutive days; 21 days later the same dosage of GM-CSF was administered i.p. for consecutive days. Four dosage levels were studied: 1, 2, 4, and 8 μg/kg/day. GM-CSF was well tolerated after i.v. and i.p. administration at doses up to 8 μ/kg/day. A transient fall followed by an elevation of circulation white cells was observed over a 24-h period after both i.v. and i.p. GM-CSF administration (mean minimum ± SE as % baseline): 38 ± 8% at 30 min after i.v. administration, 21 ± 5% at 60 min after i.p. administration; mean maximum: 220 ± 41% at 6 h after i.v. administration, 202 ± at 12 h after i.p. administration). The magnitude and time course of these changes were very similar for the two routes despite an up to 400-fold difference in serum GM-CSF levels at the same time points. Changes in leukocyte count and differential and neutrophil function were also similar over the 3-week period after both i.v. and i.p. administration. In the only levels of GM-CSf in peritoneal fluid (Cmax343 ng/ml) with maintenance of high concerntrations over 24 h (C24h128 ng/ml). In a second patient who had i.p. fulid accessible, a dramatic increase (∼100-fold) in the number of neutophils in peritoneal fluid was observed after i.p. GM-CSF. Thus, GM-CSF can be administered safely into the peritoneal cavity. Intraperitoneal administration has similar slystemic effects to i.v. administration but may have additional local effects, such as attraction into the peritoneal cavity and activation of neutophils. Further exploration of i.p. GM-CSF in the mangement of intraperitoneal infection and malignancy is warranted.

Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalJournal of Immunotherapy
Volume15
Issue number1
DOIs
StatePublished - Jan 1994
Externally publishedYes

Keywords

  • Abdominal neoplasm
  • Cytokine
  • Granulocytemacrophage colony-stimulating factor
  • Intraperitoneal infusions

Fingerprint

Dive into the research topics of 'Phase I trial of intravenous and intraperitoneal administration of granulocyte-macrophage colony-stimulting factor'. Together they form a unique fingerprint.

Cite this