Pharmacological characterization of β-endorphin- and dynorphin A1-17-induced feeding using G-protein α-subunit antisense probes in rats

Robert M. Silva; Henya C. Grossman; Grace C. Rossi; Gavril W. Pasternak; Richard J. Bodnar

doi:10.1016/S0196-9781(02)00036-0

Pharmacological characterization of β-endorphin- and dynorphin A_1-17-induced feeding using G-protein α-subunit antisense probes in rats

Robert M. Silva, Henya C. Grossman, Grace C. Rossi, Gavril W. Pasternak, Richard J. Bodnar

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Antisense (AS) oligodeoxynucleotides targeting G-protein α-subunits distinguish feeding responses of morphine and its metabolite, as well as nocturnal and deprivation-induced feeding. The present study examined whether feeding elicited by β-endorphin (βEND) or dynorphin A_1-17 was altered by ventricularly-applied G_iα₁, G_iα₂, G_iα₃, G_sα, G_oα, G_qα or G_x/zα AS probes, or a nonsense (NS) control. The βEND-induced feeding was reduced by the G_iα₁ and G_x/zα AS probes, and increased by G_iα₂ or G_iα₃ AS treatment. Dynorphin-induced feeding was attenuated by G_iα₁ and G_oα AS treatment. Yet, G_sα or G_qα AS and NS treatments failed to alter opioid agonist-induced feeding. These data provide initial characterization of potential effector signaling pathways mediating βEND and dynorphin-induced feeding.

Original language	English
Pages (from-to)	1101-1106
Number of pages	6
Journal	Peptides
Volume	23
Issue number	6
DOIs	https://doi.org/10.1016/S0196-9781(02)00036-0
State	Published - 2002
Externally published	Yes

Keywords

Dynorphin A
Feeding
G-protein α-subunit antisense
β-Endorphin

Access to Document

10.1016/S0196-9781(02)00036-0

Cite this

@article{835daea2b96e4b139896d0c4034f224c,

title = "Pharmacological characterization of β-endorphin- and dynorphin A1-17-induced feeding using G-protein α-subunit antisense probes in rats",

abstract = "Antisense (AS) oligodeoxynucleotides targeting G-protein α-subunits distinguish feeding responses of morphine and its metabolite, as well as nocturnal and deprivation-induced feeding. The present study examined whether feeding elicited by β-endorphin (βEND) or dynorphin A1-17 was altered by ventricularly-applied Giα1, Giα2, Giα3, Gsα, Goα, Gqα or Gx/zα AS probes, or a nonsense (NS) control. The βEND-induced feeding was reduced by the Giα1 and Gx/zα AS probes, and increased by Giα2 or Giα3 AS treatment. Dynorphin-induced feeding was attenuated by Giα1 and Goα AS treatment. Yet, Gsα or Gqα AS and NS treatments failed to alter opioid agonist-induced feeding. These data provide initial characterization of potential effector signaling pathways mediating βEND and dynorphin-induced feeding.",

keywords = "Dynorphin A, Feeding, G-protein α-subunit antisense, β-Endorphin",

author = "Silva, \{Robert M.\} and Grossman, \{Henya C.\} and Rossi, \{Grace C.\} and Pasternak, \{Gavril W.\} and Bodnar, \{Richard J.\}",

note = "Funding Information: This research was supported in part from National Science Foundation Grant IBN98-16699 (R.J.B.); National Institute of Drug Abuse Grants DA07274 (G.W.P.), DA00220 (G.W.P.) and DA00310 (G.C.R.), City University of New York Science Fellowship (R.M.S.), and Queens College Howard Hughes Medical Institute Summer Program for Undergraduate Research (H.C.G.).",

year = "2002",

doi = "10.1016/S0196-9781(02)00036-0",

language = "English",

volume = "23",

pages = "1101--1106",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "6",

}

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T1 - Pharmacological characterization of β-endorphin- and dynorphin A1-17-induced feeding using G-protein α-subunit antisense probes in rats

AU - Silva, Robert M.

AU - Grossman, Henya C.

AU - Rossi, Grace C.

AU - Pasternak, Gavril W.

AU - Bodnar, Richard J.

N1 - Funding Information: This research was supported in part from National Science Foundation Grant IBN98-16699 (R.J.B.); National Institute of Drug Abuse Grants DA07274 (G.W.P.), DA00220 (G.W.P.) and DA00310 (G.C.R.), City University of New York Science Fellowship (R.M.S.), and Queens College Howard Hughes Medical Institute Summer Program for Undergraduate Research (H.C.G.).

PY - 2002

Y1 - 2002

N2 - Antisense (AS) oligodeoxynucleotides targeting G-protein α-subunits distinguish feeding responses of morphine and its metabolite, as well as nocturnal and deprivation-induced feeding. The present study examined whether feeding elicited by β-endorphin (βEND) or dynorphin A1-17 was altered by ventricularly-applied Giα1, Giα2, Giα3, Gsα, Goα, Gqα or Gx/zα AS probes, or a nonsense (NS) control. The βEND-induced feeding was reduced by the Giα1 and Gx/zα AS probes, and increased by Giα2 or Giα3 AS treatment. Dynorphin-induced feeding was attenuated by Giα1 and Goα AS treatment. Yet, Gsα or Gqα AS and NS treatments failed to alter opioid agonist-induced feeding. These data provide initial characterization of potential effector signaling pathways mediating βEND and dynorphin-induced feeding.

AB - Antisense (AS) oligodeoxynucleotides targeting G-protein α-subunits distinguish feeding responses of morphine and its metabolite, as well as nocturnal and deprivation-induced feeding. The present study examined whether feeding elicited by β-endorphin (βEND) or dynorphin A1-17 was altered by ventricularly-applied Giα1, Giα2, Giα3, Gsα, Goα, Gqα or Gx/zα AS probes, or a nonsense (NS) control. The βEND-induced feeding was reduced by the Giα1 and Gx/zα AS probes, and increased by Giα2 or Giα3 AS treatment. Dynorphin-induced feeding was attenuated by Giα1 and Goα AS treatment. Yet, Gsα or Gqα AS and NS treatments failed to alter opioid agonist-induced feeding. These data provide initial characterization of potential effector signaling pathways mediating βEND and dynorphin-induced feeding.

KW - Dynorphin A

KW - Feeding

KW - G-protein α-subunit antisense

KW - β-Endorphin

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