Phagocytosis imprints heterogeneity in tissue-resident macrophages

Noelia A-Gonzalez; Juan A. Quintana; Susana García-Silva; Marina Mazariegos; Arturo González de la Aleja; José A. Nicolás-ávila; Wencke Walter; Jose M. Adrover; Georgiana Crainiciuc; Vijay K. Kuchroo; Carla V. Rothlin; Héctor Peinado; Antonio Castrillo; Mercedes Ricote; Andrés Hidalgo

doi:10.1084/jem.20161375

Phagocytosis imprints heterogeneity in tissue-resident macrophages

Noelia A-Gonzalez, Juan A. Quintana, Susana García-Silva, Marina Mazariegos, Arturo González de la Aleja, José A. Nicolás-ávila, Wencke Walter, Jose M. Adrover, Georgiana Crainiciuc, Vijay K. Kuchroo, Carla V. Rothlin, Héctor Peinado, Antonio Castrillo, Mercedes Ricote, Andrés Hidalgo

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201 Scopus citations

Abstract

Tissue-resident macrophages display varying phenotypic and functional properties that are largely specified by their local environment. One of these functions, phagocytosis, mediates the natural disposal of billions of cells, but its mechanisms and consequences within living tissues are poorly defined. Using a parabiosis-based strategy, we identified and isolated macrophages from multiple tissues as they phagocytosed blood-borne cellular material. Phagocytosis was circadianally regulated and mediated by distinct repertoires of receptors, opsonins, and transcription factors in macrophages from each tissue. Although the tissue of residence defined the core signature of macrophages, phagocytosis imprinted a distinct antiinflammatory profile. Phagocytic macrophages expressed CD206, displayed blunted expression of Il1b, and supported tissue homeostasis. Thus, phagocytosis is a source of macrophage heterogeneity that acts together with tissue-derived factors to preserve homeostasis.

Original language	English
Pages (from-to)	1281-1296
Number of pages	16
Journal	Journal of Experimental Medicine
Volume	214
Issue number	5
DOIs	https://doi.org/10.1084/jem.20161375
State	Published - 1 May 2017
Externally published	Yes

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A-Gonzalez, N., Quintana, J. A., García-Silva, S., Mazariegos, M., de la Aleja, A. G., Nicolás-ávila, J. A., Walter, W., Adrover, J. M., Crainiciuc, G., Kuchroo, V. K., Rothlin, C. V., Peinado, H., Castrillo, A., Ricote, M., & Hidalgo, A. (2017). Phagocytosis imprints heterogeneity in tissue-resident macrophages. Journal of Experimental Medicine, 214(5), 1281-1296. https://doi.org/10.1084/jem.20161375

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title = "Phagocytosis imprints heterogeneity in tissue-resident macrophages",

abstract = "Tissue-resident macrophages display varying phenotypic and functional properties that are largely specified by their local environment. One of these functions, phagocytosis, mediates the natural disposal of billions of cells, but its mechanisms and consequences within living tissues are poorly defined. Using a parabiosis-based strategy, we identified and isolated macrophages from multiple tissues as they phagocytosed blood-borne cellular material. Phagocytosis was circadianally regulated and mediated by distinct repertoires of receptors, opsonins, and transcription factors in macrophages from each tissue. Although the tissue of residence defined the core signature of macrophages, phagocytosis imprinted a distinct antiinflammatory profile. Phagocytic macrophages expressed CD206, displayed blunted expression of Il1b, and supported tissue homeostasis. Thus, phagocytosis is a source of macrophage heterogeneity that acts together with tissue-derived factors to preserve homeostasis.",

author = "Noelia A-Gonzalez and Quintana, {Juan A.} and Susana Garc{\'i}a-Silva and Marina Mazariegos and {de la Aleja}, {Arturo Gonz{\'a}lez} and Nicol{\'a}s-{\'a}vila, {Jos{\'e} A.} and Wencke Walter and Adrover, {Jose M.} and Georgiana Crainiciuc and Kuchroo, {Vijay K.} and Rothlin, {Carla V.} and H{\'e}ctor Peinado and Antonio Castrillo and Mercedes Ricote and Andr{\'e}s Hidalgo",

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A-Gonzalez, N, Quintana, JA, García-Silva, S, Mazariegos, M, de la Aleja, AG, Nicolás-ávila, JA, Walter, W, Adrover, JM, Crainiciuc, G, Kuchroo, VK, Rothlin, CV, Peinado, H, Castrillo, A, Ricote, M & Hidalgo, A 2017, 'Phagocytosis imprints heterogeneity in tissue-resident macrophages', Journal of Experimental Medicine, vol. 214, no. 5, pp. 1281-1296. https://doi.org/10.1084/jem.20161375

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T1 - Phagocytosis imprints heterogeneity in tissue-resident macrophages

AU - A-Gonzalez, Noelia

AU - Quintana, Juan A.

AU - García-Silva, Susana

AU - Mazariegos, Marina

AU - de la Aleja, Arturo González

AU - Nicolás-ávila, José A.

AU - Walter, Wencke

AU - Adrover, Jose M.

AU - Crainiciuc, Georgiana

AU - Kuchroo, Vijay K.

AU - Rothlin, Carla V.

AU - Peinado, Héctor

AU - Castrillo, Antonio

AU - Ricote, Mercedes

AU - Hidalgo, Andrés

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Tissue-resident macrophages display varying phenotypic and functional properties that are largely specified by their local environment. One of these functions, phagocytosis, mediates the natural disposal of billions of cells, but its mechanisms and consequences within living tissues are poorly defined. Using a parabiosis-based strategy, we identified and isolated macrophages from multiple tissues as they phagocytosed blood-borne cellular material. Phagocytosis was circadianally regulated and mediated by distinct repertoires of receptors, opsonins, and transcription factors in macrophages from each tissue. Although the tissue of residence defined the core signature of macrophages, phagocytosis imprinted a distinct antiinflammatory profile. Phagocytic macrophages expressed CD206, displayed blunted expression of Il1b, and supported tissue homeostasis. Thus, phagocytosis is a source of macrophage heterogeneity that acts together with tissue-derived factors to preserve homeostasis.

AB - Tissue-resident macrophages display varying phenotypic and functional properties that are largely specified by their local environment. One of these functions, phagocytosis, mediates the natural disposal of billions of cells, but its mechanisms and consequences within living tissues are poorly defined. Using a parabiosis-based strategy, we identified and isolated macrophages from multiple tissues as they phagocytosed blood-borne cellular material. Phagocytosis was circadianally regulated and mediated by distinct repertoires of receptors, opsonins, and transcription factors in macrophages from each tissue. Although the tissue of residence defined the core signature of macrophages, phagocytosis imprinted a distinct antiinflammatory profile. Phagocytic macrophages expressed CD206, displayed blunted expression of Il1b, and supported tissue homeostasis. Thus, phagocytosis is a source of macrophage heterogeneity that acts together with tissue-derived factors to preserve homeostasis.

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DO - 10.1084/jem.20161375

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VL - 214

SP - 1281

EP - 1296

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

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ER -

Phagocytosis imprints heterogeneity in tissue-resident macrophages

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