pH but not hypoxia affects neonatal gene expression: Relevance for housekeeping gene selection

Jill L. Maron; Michelle A. Arya; Kimberly J. Seefeld; Inga Peter; Diana W. Bianchi; Kirby L. Johnson

doi:10.1080/14767050802104843

pH but not hypoxia affects neonatal gene expression: Relevance for housekeeping gene selection

Jill L. Maron, Michelle A. Arya, Kimberly J. Seefeld, Inga Peter, Diana W. Bianchi, Kirby L. Johnson

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Objective. To identify a candidate neonatal housekeeping gene and to determine the effects of pH and PaO2 on the stability of newborn gene expression in physiologically hypoxic and acidotic newborn blood. Methods. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) amplification was performed for four commonly used housekeeping genes (GAPDH, β-actin, cyclophilin, 28S rRNA) on extracted RNA. Blood gas analyses determined pH and PaO2 levels. Results and conclusions. β-Actin was the least variable and GAPDH the most variable housekeeping gene studied. pH negatively correlated with gene expression levels. PaO2 levels did not significantly affect gene expression. These results inform selection of housekeeping genes for neonatal mRNA research.

Original language	English
Pages (from-to)	443-447
Number of pages	5
Journal	Journal of Maternal-Fetal and Neonatal Medicine
Volume	21
Issue number	7
DOIs	https://doi.org/10.1080/14767050802104843
State	Published - 2008
Externally published	Yes

Keywords

Housekeeping gene
Newborn gene expression
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)

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10.1080/14767050802104843

Cite this

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title = "pH but not hypoxia affects neonatal gene expression: Relevance for housekeeping gene selection",

abstract = "Objective. To identify a candidate neonatal housekeeping gene and to determine the effects of pH and PaO2 on the stability of newborn gene expression in physiologically hypoxic and acidotic newborn blood. Methods. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) amplification was performed for four commonly used housekeeping genes (GAPDH, β-actin, cyclophilin, 28S rRNA) on extracted RNA. Blood gas analyses determined pH and PaO2 levels. Results and conclusions. β-Actin was the least variable and GAPDH the most variable housekeeping gene studied. pH negatively correlated with gene expression levels. PaO2 levels did not significantly affect gene expression. These results inform selection of housekeeping genes for neonatal mRNA research.",

keywords = "Housekeeping gene, Newborn gene expression, Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)",

author = "Maron, {Jill L.} and Arya, {Michelle A.} and Seefeld, {Kimberly J.} and Inga Peter and Bianchi, {Diana W.} and Johnson, {Kirby L.}",

note = "Funding Information: We would like to thank Helene Stroh and the Departments of Respiratory Therapy and Obstetrics and Gynecology at Tufts Medical Center, Boston, MA, USA for their contribution to this work. This work was supported by an NICHD grant R01-HD42053-05 awarded to Dr Diana Bianchi and the Susan B. Saltonstall Foundation.",

year = "2008",

doi = "10.1080/14767050802104843",

language = "English",

volume = "21",

pages = "443--447",

journal = "Journal of Maternal-Fetal and Neonatal Medicine",

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TY - JOUR

T1 - pH but not hypoxia affects neonatal gene expression

T2 - Relevance for housekeeping gene selection

AU - Maron, Jill L.

AU - Arya, Michelle A.

AU - Seefeld, Kimberly J.

AU - Peter, Inga

AU - Bianchi, Diana W.

AU - Johnson, Kirby L.

N1 - Funding Information: We would like to thank Helene Stroh and the Departments of Respiratory Therapy and Obstetrics and Gynecology at Tufts Medical Center, Boston, MA, USA for their contribution to this work. This work was supported by an NICHD grant R01-HD42053-05 awarded to Dr Diana Bianchi and the Susan B. Saltonstall Foundation.

PY - 2008

Y1 - 2008

N2 - Objective. To identify a candidate neonatal housekeeping gene and to determine the effects of pH and PaO2 on the stability of newborn gene expression in physiologically hypoxic and acidotic newborn blood. Methods. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) amplification was performed for four commonly used housekeeping genes (GAPDH, β-actin, cyclophilin, 28S rRNA) on extracted RNA. Blood gas analyses determined pH and PaO2 levels. Results and conclusions. β-Actin was the least variable and GAPDH the most variable housekeeping gene studied. pH negatively correlated with gene expression levels. PaO2 levels did not significantly affect gene expression. These results inform selection of housekeeping genes for neonatal mRNA research.

AB - Objective. To identify a candidate neonatal housekeeping gene and to determine the effects of pH and PaO2 on the stability of newborn gene expression in physiologically hypoxic and acidotic newborn blood. Methods. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) amplification was performed for four commonly used housekeeping genes (GAPDH, β-actin, cyclophilin, 28S rRNA) on extracted RNA. Blood gas analyses determined pH and PaO2 levels. Results and conclusions. β-Actin was the least variable and GAPDH the most variable housekeeping gene studied. pH negatively correlated with gene expression levels. PaO2 levels did not significantly affect gene expression. These results inform selection of housekeeping genes for neonatal mRNA research.

KW - Housekeeping gene

KW - Newborn gene expression

KW - Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)

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DO - 10.1080/14767050802104843

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AN - SCOPUS:47349120494

SN - 1476-7058

VL - 21

SP - 443

EP - 447

JO - Journal of Maternal-Fetal and Neonatal Medicine

JF - Journal of Maternal-Fetal and Neonatal Medicine

IS - 7

ER -

pH but not hypoxia affects neonatal gene expression: Relevance for housekeeping gene selection

Abstract

Keywords

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