Perspectives on protein crystallisation

Takashi Ochi; Victor M. Bolanos-Garcia; Vivian Stojanoff; Abel Moreno

doi:10.1016/j.pbiomolbio.2009.12.001

Perspectives on protein crystallisation

Takashi Ochi
, Victor M. Bolanos-Garcia
, Vivian Stojanoff
, Abel Moreno

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

This final part on 'perspectives' is focused on new strategies that can be used to crystallise proteins and improve the crystal quality of macromolecular complexes using any of the methods reviewed in this focused issue. Some advantages and disadvantages, limitations, and plausible applications to high-resolution X-ray crystallography are discussed.

Original language	English
Pages (from-to)	56-63
Number of pages	8
Journal	Progress in Biophysics and Molecular Biology
Volume	101
Issue number	1-3
DOIs	https://doi.org/10.1016/j.pbiomolbio.2009.12.001
State	Published - Nov 2009
Externally published	Yes

Keywords

Counter-diffusion
Crystallisation
DNA-repair proteins
Drug discovery
Gel
Lif1p-Lig4p
Protein crystal growth

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10.1016/j.pbiomolbio.2009.12.001

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title = "Perspectives on protein crystallisation",

abstract = "This final part on 'perspectives' is focused on new strategies that can be used to crystallise proteins and improve the crystal quality of macromolecular complexes using any of the methods reviewed in this focused issue. Some advantages and disadvantages, limitations, and plausible applications to high-resolution X-ray crystallography are discussed.",

keywords = "Counter-diffusion, Crystallisation, DNA-repair proteins, Drug discovery, Gel, Lif1p-Lig4p, Protein crystal growth",

author = "Takashi Ochi and Bolanos-Garcia, \{Victor M.\} and Vivian Stojanoff and Abel Moreno",

note = "Funding Information: TO would like to thank Semin Lee for helping the analysis of the PQS database. One of the authors (AM) acknowledges the financial support from CONACYT, project No. 91999 and travelling expenses from DGAPA-UNAM during the sabbatical time at the University of Cambridge as a visiting professor. All authors acknowledge to Dr. Carlo Petosa for the support during X-ray data collection of DNA-repair proteins on beamline ID14-4 at ESRF, also to Prof. Sir Tom L. Blundell for stimulating suggestions for the edition of this focused issue.",

year = "2009",

month = nov,

doi = "10.1016/j.pbiomolbio.2009.12.001",

language = "English",

volume = "101",

pages = "56--63",

journal = "Progress in Biophysics and Molecular Biology",

issn = "0079-6107",

publisher = "Elsevier Ltd.",

number = "1-3",

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T1 - Perspectives on protein crystallisation

AU - Ochi, Takashi

AU - Bolanos-Garcia, Victor M.

AU - Stojanoff, Vivian

AU - Moreno, Abel

N1 - Funding Information: TO would like to thank Semin Lee for helping the analysis of the PQS database. One of the authors (AM) acknowledges the financial support from CONACYT, project No. 91999 and travelling expenses from DGAPA-UNAM during the sabbatical time at the University of Cambridge as a visiting professor. All authors acknowledge to Dr. Carlo Petosa for the support during X-ray data collection of DNA-repair proteins on beamline ID14-4 at ESRF, also to Prof. Sir Tom L. Blundell for stimulating suggestions for the edition of this focused issue.

PY - 2009/11

Y1 - 2009/11

N2 - This final part on 'perspectives' is focused on new strategies that can be used to crystallise proteins and improve the crystal quality of macromolecular complexes using any of the methods reviewed in this focused issue. Some advantages and disadvantages, limitations, and plausible applications to high-resolution X-ray crystallography are discussed.

AB - This final part on 'perspectives' is focused on new strategies that can be used to crystallise proteins and improve the crystal quality of macromolecular complexes using any of the methods reviewed in this focused issue. Some advantages and disadvantages, limitations, and plausible applications to high-resolution X-ray crystallography are discussed.

KW - Counter-diffusion

KW - Crystallisation

KW - DNA-repair proteins

KW - Drug discovery

KW - Gel

KW - Lif1p-Lig4p

KW - Protein crystal growth

UR - https://www.scopus.com/pages/publications/75249094295

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DO - 10.1016/j.pbiomolbio.2009.12.001

M3 - Article

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AN - SCOPUS:75249094295

SN - 0079-6107

VL - 101

SP - 56

EP - 63

JO - Progress in Biophysics and Molecular Biology

JF - Progress in Biophysics and Molecular Biology

IS - 1-3

ER -

Perspectives on protein crystallisation

Abstract

Keywords

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