Persistence of tumor DNA in plasma of breast cancer patients after mastectomy

Jose M. Silva, Jose M. Garcia, Gemma Dominguez, Javier Silva, Celia Miralles, Blanca Cantos, Santiago Coca, Mariano Provencio, Pilar España, Felix Bonilla

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Background: We investigated tumor DNA changes before and after mastectomy in the plasma of breast cancer patients with no disseminated disease and eventually investigated these changes' relationship to specific pathological parameters of the tumors. Methods: We studied 41 patients. DNA extracted from tumor and normal breast tissues, mononuclear blood cells, and plasma was used for molecular studies. Alterations in the microsatellite markers D17S855, D17S654, D16S421, TH2, D10S197, and D9S161, as well as point mutations in the p53 gene and aberrant methylation of p16INK4a, were used to identify and characterize tumor and plasma DNA. A number of tumor clinicopathological parameters were analyzed in each patient. Results: We found that 18 (44%) of the 27 patients with alterations in tumor DNA presented the same plasma DNA alteration before mastectomy, and persistence of the same molecular features was detected in plasma DNA 4 to 6 weeks postmastectomy in 8 (19.5%) patients. Patients with vascular invasion, more than three lymph node metastases, and higher histological grade at diagnosis displayed plasma DNA after mastectomy with a significant difference. Conclusions: Persistence of plasma DNA with features of tumor DNA may be present after mastectomy in breast cancer patients, and its relation to bad-prognosis histological parameters may suggest undetectable micrometastatic disease.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalAnnals of Surgical Oncology
Volume9
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • Circulating DNA
  • Genetic markers
  • Mammary malignancies
  • Poor prognosis
  • Postmastectomy

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