Peripapillary nerve fiber layer thickness measurement reproducibility using scanning laser polarimetry

Sek Tien Hoh, Hiroshi Ishikawa, David S. Greenfield, Jeffrey M. Liebmann, Sek Jin Chew, Robert Ritch

Research output: Contribution to journalArticlepeer-review

99 Scopus citations


Purpose: The authors assess the reproducibility of retinal nerve fiber layer (RNFL) thickness measurements using scanning laser polarimetry. Methods: The right eyes of five healthy individuals were scanned by two trained operators (S.T.H., H.I.) using the Nerve Fiber Analyzer II (Laser Diagnostic Technologies, Inc., San Diego, CA, U.S.A.). Each participant was scanned six consecutive times per session for five sessions carried out over separate days within a two-week period. Intra- and interoperator measurement reproducibility was assessed. Results: Intraoperator reproducibility was high. Mean coefficients of variation for total RNFL thickness measurements were 4.48 ± 1.76% and 4.92 ± 2.32% for operators 1 and 2, respectively. Interoperator reproducibility was high (p = 0.20-0.93) if a single ellipse was applied to the images of both examiners. However, measurement reproducibility worsened if each operator created his own measurement ellipse (p ≤ 0.05 for 3 out of 5 participants). Conclusion: Interoperator error in image analysis can be minimized by using a single ellipse for the baseline and all subsequent scans even if subsequent scans are acquired by different operators.

Original languageEnglish
Pages (from-to)12-15
Number of pages4
JournalJournal of Glaucoma
Issue number1
StatePublished - Feb 1998
Externally publishedYes


  • Birefringence
  • Glaucoma
  • Imaging
  • Nerve fiber layer
  • Optic nerve
  • Repeatability
  • Reproducibility
  • Scanning laser polarimetry


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