PDGF-C is a new protease-activated ligand for the PDGF α-receptor

Xuri Li; Annica Pontén; Karin Aase; Linda Karlsson; Alexandra Abramsson; Marko Uutela; Gudrun Bäckström; Mats Hellström; Hans Boström; Hong Li; Philippe Soriano; Christer Betsholtz; Carl Henrik Heldin; Kari Alitalo; Arne Östman; Ulf Eriksson

doi:10.1038/35010579

PDGF-C is a new protease-activated ligand for the PDGF α-receptor

Xuri Li
, Annica Pontén
, Karin Aase
, Linda Karlsson
, Alexandra Abramsson
, Marko Uutela
, Gudrun Bäckström
, Mats Hellström
, Hans Boström
, Hong Li
, Philippe Soriano
, Christer Betsholtz
, Carl Henrik Heldin
, Kari Alitalo
, Arne Östman
, Ulf Eriksson

Research output: Contribution to journal › Article › peer-review

537 Scopus citations

Abstract

Platelet-derived growth factors (PDGFs) are important in many types of mesenchymal cell. Here we identify a new PDGF, PDGF-C, which binds to and activates the PDGF α-receptor. PDGF-C is activated by proteolysis and induces proliferation of fibroblasts when overexpressed in transgenic mice. In situ hybridization analysis in the murine embryonic kidney shows preferential expression of PDGF-C messenger RNA in the metanephric mesenchyme during epithelial conversion. Analysis of kidneys lacking the PDGF α-receptor shows selective loss of mesenchymal cells adjacent to sites of expression of PDGF-C mRNA; this is not found in kidneys from animals lacking PDGF-A or both PDGF-A and PDGF-B, indicating that PDGF-C may have a unique function.

Original language	English
Pages (from-to)	302-307
Number of pages	6
Journal	Nature Cell Biology
Volume	2
Issue number	5
DOIs	https://doi.org/10.1038/35010579
State	Published - 2000
Externally published	Yes

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@article{73ed8e8c9cd64dfc85a467cdeab2f901,

title = "PDGF-C is a new protease-activated ligand for the PDGF α-receptor",

abstract = "Platelet-derived growth factors (PDGFs) are important in many types of mesenchymal cell. Here we identify a new PDGF, PDGF-C, which binds to and activates the PDGF α-receptor. PDGF-C is activated by proteolysis and induces proliferation of fibroblasts when overexpressed in transgenic mice. In situ hybridization analysis in the murine embryonic kidney shows preferential expression of PDGF-C messenger RNA in the metanephric mesenchyme during epithelial conversion. Analysis of kidneys lacking the PDGF α-receptor shows selective loss of mesenchymal cells adjacent to sites of expression of PDGF-C mRNA; this is not found in kidneys from animals lacking PDGF-A or both PDGF-A and PDGF-B, indicating that PDGF-C may have a unique function.",

author = "Xuri Li and Annica Pont{\'e}n and Karin Aase and Linda Karlsson and Alexandra Abramsson and Marko Uutela and Gudrun B{\"a}ckstr{\"o}m and Mats Hellstr{\"o}m and Hans Bostr{\"o}m and Hong Li and Philippe Soriano and Christer Betsholtz and Heldin, \{Carl Henrik\} and Kari Alitalo and Arne {\"O}stman and Ulf Eriksson",

note = "Funding Information: ACKNOWLEDGEMENTS We thank B. {\AA}kerblom and C. Raynoschek for technical assistance, and U. Engstr{\"o}m for synthesizing peptides. This study was supported by grants from the Swedish and Finnish Cancer Societies, the Swedish Medical Research Council, the G{\"o}ran Gustafsson Foundation, the Inga-Britt and Arne Lundberg Foundation, the Swedish Heart and Lung Foundation and the Novo Nordisk Foundation. The cooperative research agreement between the Ludwig Institute for Cancer Research, Stockholm branch, and Fuji Photo Film (Europe) is gratefully acknowledged. Correspondence and requests for materials should be addressed to U.E. The nucleotide and amino-acid sequences of human PDGF-C have been deposited at GenBank under accession number AF244813.",

year = "2000",

doi = "10.1038/35010579",

language = "English",

volume = "2",

pages = "302--307",

journal = "Nature Cell Biology",

issn = "1465-7392",

publisher = "Nature Research",

number = "5",

}

TY - JOUR

T1 - PDGF-C is a new protease-activated ligand for the PDGF α-receptor

AU - Li, Xuri

AU - Pontén, Annica

AU - Aase, Karin

AU - Karlsson, Linda

AU - Abramsson, Alexandra

AU - Uutela, Marko

AU - Bäckström, Gudrun

AU - Hellström, Mats

AU - Boström, Hans

AU - Li, Hong

AU - Soriano, Philippe

AU - Betsholtz, Christer

AU - Heldin, Carl Henrik

AU - Alitalo, Kari

AU - Östman, Arne

AU - Eriksson, Ulf

N1 - Funding Information: ACKNOWLEDGEMENTS We thank B. Åkerblom and C. Raynoschek for technical assistance, and U. Engström for synthesizing peptides. This study was supported by grants from the Swedish and Finnish Cancer Societies, the Swedish Medical Research Council, the Göran Gustafsson Foundation, the Inga-Britt and Arne Lundberg Foundation, the Swedish Heart and Lung Foundation and the Novo Nordisk Foundation. The cooperative research agreement between the Ludwig Institute for Cancer Research, Stockholm branch, and Fuji Photo Film (Europe) is gratefully acknowledged. Correspondence and requests for materials should be addressed to U.E. The nucleotide and amino-acid sequences of human PDGF-C have been deposited at GenBank under accession number AF244813.

PY - 2000

Y1 - 2000

N2 - Platelet-derived growth factors (PDGFs) are important in many types of mesenchymal cell. Here we identify a new PDGF, PDGF-C, which binds to and activates the PDGF α-receptor. PDGF-C is activated by proteolysis and induces proliferation of fibroblasts when overexpressed in transgenic mice. In situ hybridization analysis in the murine embryonic kidney shows preferential expression of PDGF-C messenger RNA in the metanephric mesenchyme during epithelial conversion. Analysis of kidneys lacking the PDGF α-receptor shows selective loss of mesenchymal cells adjacent to sites of expression of PDGF-C mRNA; this is not found in kidneys from animals lacking PDGF-A or both PDGF-A and PDGF-B, indicating that PDGF-C may have a unique function.

AB - Platelet-derived growth factors (PDGFs) are important in many types of mesenchymal cell. Here we identify a new PDGF, PDGF-C, which binds to and activates the PDGF α-receptor. PDGF-C is activated by proteolysis and induces proliferation of fibroblasts when overexpressed in transgenic mice. In situ hybridization analysis in the murine embryonic kidney shows preferential expression of PDGF-C messenger RNA in the metanephric mesenchyme during epithelial conversion. Analysis of kidneys lacking the PDGF α-receptor shows selective loss of mesenchymal cells adjacent to sites of expression of PDGF-C mRNA; this is not found in kidneys from animals lacking PDGF-A or both PDGF-A and PDGF-B, indicating that PDGF-C may have a unique function.

UR - https://www.scopus.com/pages/publications/0033776193

U2 - 10.1038/35010579

DO - 10.1038/35010579

M3 - Article

C2 - 10806482

AN - SCOPUS:0033776193

SN - 1465-7392

VL - 2

SP - 302

EP - 307

JO - Nature Cell Biology

JF - Nature Cell Biology

IS - 5

ER -

PDGF-C is a new protease-activated ligand for the PDGF α-receptor

Abstract

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